Lancaster W D, Olson C, Meinke W
Proc Natl Acad Sci U S A. 1977 Feb;74(2):524-8. doi: 10.1073/pnas.74.2.524.
Four of five spontaneous benign equine connective tissue tumors of unknown etiology and a bovine papilloma virus (BPV)-induced equine tumor contained BPV-specific DNA sequences as determined by DNA-DNA hybridization of DNA from tumors with BPV DNA labeled in vitro. Analysis of the kinetics of reassociation indicated that 20-75% of the BPV genome was present in the various tumors. The number of partial BPV genome equivalents ranged from 60 to 500 copies per diploid quantity of cellular DNA. Thermal denaturation profiles of duplexes formed between labeled BPV DNA and DNA from tumor cells indicated two tumors contained viral DNA with base sequences identical to BPV DNA. Three tumors (including DNA from the BPV-induced tumor) contained BPV-related DNA sequences that were less thermally stable. The decrease in thermal denaturation temperature may be due to the presence of (adenine + thymine)-rich regions of the BPV genome in the tumor cells.
通过用体外标记的牛乳头瘤病毒(BPV)DNA与肿瘤DNA进行DNA-DNA杂交测定,5例病因不明的自发性良性马结缔组织肿瘤中有4例以及1例BPV诱导的马肿瘤含有BPV特异性DNA序列。对复性动力学的分析表明,各种肿瘤中存在20%-75%的BPV基因组。每二倍体数量的细胞DNA中,部分BPV基因组当量的数量范围为60至500个拷贝。标记的BPV DNA与肿瘤细胞DNA形成的双链体的热变性图谱表明,有2例肿瘤含有与BPV DNA碱基序列相同的病毒DNA。3例肿瘤(包括来自BPV诱导肿瘤的DNA)含有热稳定性较差但与BPV相关的DNA序列。热变性温度的降低可能是由于肿瘤细胞中BPV基因组富含(腺嘌呤+胸腺嘧啶)区域。