1型和2型牛乳头瘤病毒DNA与病毒诱导的仓鼠肿瘤及自然发生的马肿瘤的DNA杂交。

Hybridization of bovine papilloma virus type 1 and type 2 DNA to DNA from virus-induced hamster tumors and naturally occurring equine tumors.

作者信息

Lancaster W D, Theilen G H, Olson C

出版信息

Intervirology. 1979;11(4):227-33. doi: 10.1159/000149038.

Abstract

DNAs from bovine papilloma virus(BPV)-induced hamster tumors and from equine connective tissue tumors of unknown etiology were examined for BPV DNA sequences by molecular hybridization. DNA from two distinct classes of BPV (type 1 and type 2) was labeled in vitro and used as probes. Analysis of DNA-DNA reassociation kinetics indicated that both virus types were capable of tumor induction in the hamster. DNA isolated from 6 of 7 equine tumors accelerated the reassociation of the BPV DNA probes. BPV type 1 or type 2 DNA hybridized extensively to DNA from 3 tumors, while 3 other tumors contained DNA sequences to which only a portion of the probes hybridized. Partial hybridization of probe DNAs to tumor DNA suggested the possible existence of a third BPV class.

摘要

通过分子杂交检测来自牛乳头瘤病毒（BPV）诱导的仓鼠肿瘤以及病因不明的马结缔组织肿瘤的DNA中的BPV DNA序列。来自两种不同类型BPV（1型和2型）的DNA在体外进行标记并用作探针。DNA-DNA重缔合动力学分析表明，两种病毒类型都能够在仓鼠中诱导肿瘤。从7个马肿瘤中的6个分离出的DNA加速了BPV DNA探针的重缔合。1型或2型BPV DNA与3个肿瘤的DNA广泛杂交，而其他3个肿瘤包含的DNA序列仅与一部分探针杂交。探针DNA与肿瘤DNA的部分杂交表明可能存在第三种BPV类型。