Mao Shuhong, Liu Yanna, Hou Yali, Ma Xiaoyu, Yang Juanjuan, Han Haichao, Wu Jianlin, Jia Longgang, Qin Huimin, Lu Fuping
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science & Technology, Industrial Microbiology Laboratory, College of Biotechnology, Tianjin Economic and Technological Development Zone No. 29, 13 Main Street Tianjin 300457 China
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology Tianjin 300457 China.
RSC Adv. 2018 Nov 30;8(70):39897-39901. doi: 10.1039/c8ra07556e. eCollection 2018 Nov 28.
Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and l-rhamnonic acid by reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for TCCC11892, achieving a yield of over 90 g L for the first 7 cycles. The excellent performance of in the production of lactobionic acid (119 g L) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with TCCC11892 cells should facilitate their commercial applications.
由于醛糖酸在纳米技术、食品、制药和化学工业中的应用，它们正受到越来越多的关注。具有醛糖氧化活性的微生物，而非纯化的酶，被用于商业生产，但成效有限。因此，开发利用具有更高效和新颖生物催化活性的菌株来生产醛糖酸的新工艺仍然非常重要。在本研究中，发现TCCC11892是醛糖酸的高效生产者，首次报道了其生产半乳糖酸和L-鼠李糖酸。开发了TCCC11892半连续生产麦芽糖酸和乳糖酸的工艺，在前7个循环中产量超过90 g/L。当使用废弃奶酪乳清作为廉价发酵培养基时，也观察到TCCC11892在生产乳糖酸（119 g/L）方面的优异性能。扩大上述利用TCCC11892细胞生产醛糖酸的工艺规模应有助于其商业应用。
