Scherr Claudia, Simon Meinhard, Spranger Jörg, Baumgartner Stephan
Research Institute of Organic Agriculture (FiBL), Ackerstrasse, Frick, Switzerland.
Complement Ther Med. 2009 Apr;17(2):63-70. doi: 10.1016/j.ctim.2008.10.004. Epub 2009 Jan 7.
This study investigated, whether the growth rate of Lemna gibba L. (duckweed) can be influenced by the application of homeopathic potencies of gibberellic acid, kinetin, argentum nitricum, and lemna minor.
Duckweed was grown in either potencies (14x-30x, decimal steps) or water controls (unsuccussed and succussed) over seven days. Frond (leaf-like structure) growth was measured using a non-destructive image analysis system. Growth rates were calculated for three time intervals (0-7, 0-3, 3-7 days). Five to six independent, randomized and blinded experiments were analysed for each of the four tested substances. Water control experiments were performed repeatedly to test the reliability of the experimental set-up (systematic negative controls).
The systematic negative control experiments did not yield any significant effects. Hence, false positive results could be excluded. The test system had a low coefficient of variation (1.5%). Out of the four tested substances gibberellic acid had the most pronounced effect (p=0.0002, F-test) on the main outcome parameter frond growth rate (r(area) day 0-7). Potency levels 15x, 17x, 18x, 23x and 24x reduced growth rate of Lemna gibba (p<0.05 against the pooled water control, LSD test).
Lemna gibba may be considered as a suitable test organism for further studies on the efficacy of homeopathic potencies. Evidence accumulates, that adjacent potency levels may strongly differ in their biological activity. Potential consequences for therapeutical application might be worth investigating.
本研究调查了赤霉素、激动素、硝酸银和浮萍的顺势疗法药力是否会影响浮萍（Lemna gibba L.）的生长速率。
浮萍在药力（14x - 30x，十进制步长）或水对照（未震荡和震荡）中培养7天。使用无损图像分析系统测量叶状体（叶状结构）的生长。计算三个时间间隔（0 - 7天、0 - 3天、3 - 7天）的生长速率。对四种受试物质中的每一种进行了五到六个独立、随机和盲法实验。重复进行水对照实验以测试实验设置的可靠性（系统性阴性对照）。
系统性阴性对照实验未产生任何显著影响。因此，可以排除假阳性结果。测试系统的变异系数较低（1.5%）。在四种受试物质中，赤霉素对主要结果参数叶状体生长速率（0 - 7天的r(面积)）影响最为显著（p = 0.0002，F检验）。药力水平15x、17x、18x、23x和24x降低了浮萍的生长速率（与合并的水对照相比，p < 0.05，LSD检验）。
浮萍可被视为进一步研究顺势疗法药力疗效的合适测试生物。越来越多的证据表明，相邻药力水平的生物活性可能存在很大差异。其对治疗应用的潜在影响可能值得研究。
