Jäger Tim, Würtenberger Sandra, Baumgartner Stephan
Institute of Integrative Medicine, University of Witten/Herdecke, Germany.
Scientific and Regulatory Affairs, Hevert-Arzneimittel GmbH & Co. KG, Nussbaum, Germany.
Homeopathy. 2019 May;108(2):128-138. doi: 10.1055/s-0038-1676464. Epub 2019 Jan 30.
We developed a bioassay with mercury-stressed duckweed ( L.) to study potential effects of homeopathically potentised mercury(II) chloride ( []). The response of this bioassay to homeopathic treatments as a function of stress intensity was also of interest.
Duckweed was severely stressed with mercury(II) chloride for 48 hours. Afterwards plants grew in either (seven different potency levels, 24x to 30x) or water controls (unsuccussed and succussed water) for 7 days. Growth rates of the frond (leaf) area were determined using a computerised image analysis system for different time intervals between the measurements on days 0, 3 and 7. Three independent experiments with potentised each were evaluated. Additionally, three water control experiments were analysed to investigate the stability of the experimental set-up (systematic negative control [SNC] experiments). All experiments were randomised and blinded.
Unsuccussed and succussed water did not significantly differ in terms of duckweed growth rate. The SNC experiments did not yield any significant effects, providing evidence for the stability of the experimental system. Data from the two control groups and the seven treatment groups ( 24x-30x) were each pooled to increase the statistical power. Duckweed growth rates for day 0 to 3 were reduced ( < 0.05) after application of compared with the controls. Growth rates for day 3 to 7 were not influenced by the homeopathic preparations.
The present test system with L. that was severely stressed by mercury yielded evidence for specific effects of 24x to 30x, namely a growth reduction in the first time period (day 0-3). This is in contrast to former experiments with slightly arsenic-stressed duckweed, where a growth increase was observed in the second time period (day 2-6). We hypothesise that the differing results are associated with the level of stress intensity (severe versus slight).
我们开发了一种利用汞胁迫浮萍（L.）的生物测定法，以研究顺势疗法稀释的氯化汞（[]）的潜在影响。该生物测定法对顺势疗法治疗的反应作为应激强度的函数也受到关注。
浮萍用氯化汞严重胁迫48小时。之后，植物在（七个不同的稀释度水平，24x至30x）或水对照（未振荡和振荡过的水）中生长7天。使用计算机图像分析系统在第0、3和7天的不同测量时间间隔确定叶状体（叶）面积的生长速率。对每个稀释度的顺势疗法药物进行了三个独立实验进行评估。此外，分析了三个水对照实验以研究实验设置的稳定性（系统阴性对照[SNC]实验）。所有实验均进行了随机化和盲法处理。
未振荡和振荡过的水在浮萍生长速率方面没有显著差异。SNC实验没有产生任何显著影响，为实验系统的稳定性提供了证据。将两个对照组和七个治疗组（24x - 30x）的数据分别合并以提高统计效力。与对照组相比，施用顺势疗法药物后第0至3天的浮萍生长速率降低（P < 0.05）。第3至7天的生长速率不受顺势疗法制剂的影响。
目前用汞严重胁迫的浮萍（L.）测试系统证明了24x至30x的顺势疗法药物有特定影响，即在第一个时间段（第0 - 3天）生长减少。这与之前用轻度砷胁迫浮萍的实验相反，在之前的实验中观察到第二个时间段（第2 - 6天）生长增加。我们假设不同的结果与应激强度水平（严重与轻度）有关。
