Institute of Complementary Medicine KIKOM, University of Bern, Switzerland.
J Altern Complement Med. 2011 Apr;17(4):315-23. doi: 10.1089/acm.2010.0246. Epub 2011 Apr 8.
A bioassay with arsenic-stressed duckweed (Lemna gibba L.) was developed to study potentially regulative effects of homeopathic preparations. We compared potentized substances (nine different potency levels between 17 x and 33 x ) with two controls (unsuccussed and succussed water) regarding their influence on number- and area-related growth rate and color of fronds (leaves). Screening included 11 potentized substances: Arsenicum album, gibberellic acid, nosode, arsenic(V), phosphorus, Conchae, Acidum picrinicum, Argentum nitricum, Crotalus horridus, Hepar sulfuris, and Mercurius vivus naturalis.
Duckweed was stressed with arsenic(V) for 48 hours. Afterwards, plants grew in either potentized substances or water controls for 6 days. Growth rate and color of fronds were determined with a computerized image analysis system for different time intervals (days 0-2, 2-6, 0-6). A systematic negative control experiment with unsuccussed water was used to investigate the stability of the bioassay. All experiments were randomized and blinded.
Arsenicum album and nosode potencies increased frond number-related growth rate compared to controls (succussed water controls or pooled water controls [succussed and unsuccussed], p < 0.05, t test). Regarding color classification, no effects were observed.
The experimental setup with L. gibba stressed by arsenic(V) provides a valuable tool to investigate regulative effects of potentized substances. In order to verify the effects of Arsenicum album and nosode potencies, further independent replication experiments are necessary.
利用受到砷胁迫的浮萍（Lemna gibba L.）生物测定法来研究顺势疗法制剂的潜在调节作用。我们比较了经过潜能处理的物质（17×至 33×之间的 9 种不同浓度）与两种对照（未处理水和处理水）对叶片数和面积相关生长率和颜色的影响。筛选包括 11 种经过潜能处理的物质：砷（Arsenicum album）、赤霉素、鼻疽、五价砷、磷、贝壳、酸 picrinicum、硝酸银、响尾蛇、硫磺肝、和天然汞。
浮萍在砷（V）中应激 48 小时。之后，植物在经过潜能处理的物质或水对照中生长 6 天。使用计算机图像分析系统在不同时间间隔（0-2 天、2-6 天、0-6 天）测定叶片的生长率和颜色。未处理水的系统阴性对照实验用于研究生物测定的稳定性。所有实验均随机化和盲化。
与对照（处理水对照或混合水对照[处理和未处理]相比，砷（Arsenicum album）和鼻疽潜能增加了叶片数相关的生长率（p<0.05，t 检验）。关于颜色分类，未观察到任何影响。
使用受到砷（V）胁迫的浮萍（L. gibba）的实验设置提供了一种有价值的工具，可以研究经过潜能处理的物质的调节作用。为了验证砷（Arsenicum album）和鼻疽潜能的作用，需要进行进一步的独立复制实验。
