Rokeach L A, Haselby J A, Meilof J F, Smeenk R J, Unnasch T R, Greene B M, Hoch S O
Agouron Institute, La Jolla, CA 92037.
J Immunol. 1991 Nov 1;147(9):3031-9.
Anti-Ro/SS-A antibodies are commonly found in the sera of patients with Sjögren's syndrome and SLE. These antibodies also occur in the mothers of children with neonatal lupus and congenital heart block. Ro/SS-A is a ribonucleoprotein complex whose cellular function remains unknown. To study its cellular function and to characterize its immunoreactivity, we have used an oligonucleotide designed after the published amino terminal sequence of a putative 60-kDa Ro/SS-A autoantigen to isolate its cDNA. This cDNA encodes a polypeptide that is the human homologue of calreticulin, a calcium binding protein of the endoplasmatic reticulum. The encoded polypeptide also shows a 64.4% identity with RAL-1, an Ag of the river blindness pathogen Onchocerca volvulus. Contrary to the data published by other authors, our results indicate that calreticulin is not a Ro/SS-A autoantigen. Moreover, we show that anticalreticulin autoantibodies occur in the sera of patients with SLE and patients with onchocerciasis.
抗Ro/SS-A抗体常见于干燥综合征和系统性红斑狼疮患者的血清中。这些抗体也出现在新生儿狼疮和先天性心脏传导阻滞患儿的母亲体内。Ro/SS-A是一种核糖核蛋白复合体，其细胞功能尚不清楚。为了研究其细胞功能并表征其免疫反应性，我们根据已发表的假定60 kDa Ro/SS-A自身抗原的氨基末端序列设计了一种寡核苷酸，以分离其cDNA。该cDNA编码一种多肽，它是内质网钙结合蛋白钙网蛋白的人类同源物。编码的多肽与盘尾丝虫病病原体盘尾丝虫的一种抗原RAL-1也有64.4%的同一性。与其他作者发表的数据相反，我们的结果表明钙网蛋白不是Ro/SS-A自身抗原。此外，我们发现抗钙网蛋白自身抗体出现在系统性红斑狼疮患者和盘尾丝虫病患者的血清中。
