Chen Zhen, Meng Huan, Xing Gengmei, Yuan Hui, Zhao Feng, Liu Ru, Chang Xuelin, Gao Xueyun, Wang Tiancheng, Jia Guang, Ye Chang, Chai Zhifang, Zhao Yuliang
Laboratory for Bio-Environmental Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Environ Sci Technol. 2008 Dec 1;42(23):8985-92. doi: 10.1021/es800975u.
Epidemiologic studies have revealed that pollution by ambient particulates is associated with respiratory and cardiovascular diseases, particularly in older people. Toxicologic sensitivity of nanoparticles in different ages was investigated for the first time to demonstrate and explain an age-related difference in response to manufactured nanoparticles. Young, adult, and old rats physiologically inhaled air containing aerosol of manufactured SiO2 nanoparticles (24.1 mg/m3; 40 min/day) for four weeks. Changes in serum biomarkers, hemorheologic, pulmonary inflammation, heart injury, and pathology in rats of different ages and their corresponding controls were compared. Inhalation of SiO2 nanoparticles under identical conditions caused pulmonary and cardiovascular alterations in old rats, yet less change in young and adult rats, including pulmonary inflammation, myocardial ischemic damage, atrio-ventricular blockage, and increase in fibrinogen concentration and blood viscosity. Old individuals were more sensitive to nanoparticle exposure than the young and adult rats. The risk of causing pulmonary damages was: old > young > adult The risk of cardiovascular disorder was observed only in old age. Our results suggest that different ages may require different biomarkers for identifying pulmonary toxicity during inhalation of nanoparticles.
流行病学研究表明，环境颗粒物污染与呼吸道和心血管疾病有关，尤其是在老年人中。首次研究了不同年龄的纳米颗粒的毒理学敏感性，以证明和解释对人造纳米颗粒反应的年龄相关差异。幼年、成年和老年大鼠在生理状态下吸入含有二氧化硅纳米颗粒气溶胶（24.1毫克/立方米；每天40分钟）的空气，持续四周。比较了不同年龄大鼠及其相应对照组的血清生物标志物、血液流变学、肺部炎症、心脏损伤和病理学变化。在相同条件下吸入二氧化硅纳米颗粒会导致老年大鼠出现肺部和心血管改变，但幼年和成年大鼠的变化较小，包括肺部炎症、心肌缺血损伤、房室传导阻滞以及纤维蛋白原浓度和血液粘度增加。老年个体比幼年和成年大鼠对纳米颗粒暴露更敏感。造成肺部损伤的风险为：老年＞幼年＞成年。仅在老年观察到心血管疾病风险。我们的结果表明，不同年龄在吸入纳米颗粒期间识别肺部毒性可能需要不同的生物标志物。
