DNA寡聚物的动力学控制自组装

Kinetically controlled self-assembly of DNA oligomers.

作者信息

Lubrich Daniel, Green Simon J, Turberfield Andrew J

机构信息

Department of Physics, University of Oxford, South Parks Road, Oxford, OX1 3PU, UK.

出版信息

J Am Chem Soc. 2009 Feb 25;131(7):2422-3. doi: 10.1021/ja807765v.

DOI:10.1021/ja807765v

PMID:19193012

Abstract

Metastable two-stranded DNA loops can be assembled into extended DNA oligomers by kinetically controlled self-assembly. Along the designed reaction pathway, the sequence of hybridization reactions is controlled by progressively revealing toeholds required to initiate strand-displacement reactions. The product length depends inversely on seed concentration and ranges from a few hundred to several thousand base-pairs.

摘要

亚稳态双链DNA环可通过动力学控制的自组装组装成延伸的DNA寡聚物。沿着设计的反应途径，杂交反应的顺序由逐步暴露引发链置换反应所需的链端来控制。产物长度与种子浓度成反比，范围从几百到几千个碱基对。