Sakamoto Mitsuo, Suzuki Natsuko, Matsunaga Naohisa, Koshihara Kimihito, Seki Masayasu, Komiya Hideaki, Benno Yoshimi
RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2843-7. doi: 10.1099/ijs.0.010611-0. Epub 2009 Jul 23.
Three bacterial strains that had been isolated from human blood cultures, MS-1(T), MS-2 and MS-3, were characterized for their phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles and phylogenetic positions based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the isolates were members of the genus Parabacteroides. These isolates were most closely related to Parabacteroides goldsteinii JCM 13446(T), with 95.9 % 16S rRNA gene sequence similarity. The levels of sequence similarity among the three strains were 99.7-100 %. The isolates were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative and rod-shaped. The strains grew on media containing 20 % bile. These strains could be differentiated from P. goldsteinii by their ability to ferment l-arabinose and inabilities to ferment cellobiose, l-rhamnose and trehalose or to hydrolyse aesculin. The major menaquinone of the isolates was MK-10. Based on these data, we propose a novel Parabacteroides species, Parabacteroides gordonii sp. nov. The type strain is MS-1(T) (=JCM 15724(T) =CCUG 57478(T)).
从人类血液培养物中分离出的三株细菌MS-1(T)、MS-2和MS-3，基于16S rRNA基因序列分析对其表型和生化特征、细胞脂肪酸谱、甲基萘醌谱以及系统发育位置进行了表征。16S rRNA基因序列分析表明，这些分离株属于副拟杆菌属。这些分离株与戈氏副拟杆菌JCM 13446(T)关系最为密切，16S rRNA基因序列相似度为95.9%。这三株菌株之间的序列相似度水平为99.7%-100%。这些分离株为专性厌氧菌，无色素，不形成芽孢，无运动性，革兰氏阴性，杆状。这些菌株能在含20%胆汁的培养基上生长。这些菌株可通过发酵L-阿拉伯糖的能力以及不能发酵纤维二糖、L-鼠李糖和海藻糖或水解七叶苷与戈氏副拟杆菌相区分。分离株的主要甲基萘醌为MK-10。基于这些数据，我们提出一个新的副拟杆菌物种，戈登副拟杆菌（Parabacteroides gordonii sp. nov.）。模式菌株为MS-1(T)（=JCM 15724(T)=CCUG 57478(T)）。
