Kämpfer Peter, Lodders Nicole, Busse Hans-Jürgen
Institut für Angewandte Mikrobiologie, Universität Giessen, D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):341-4. doi: 10.1099/ijs.0.002675-0.
Strain TW5(T), a motile, Gram-negative bacterium that produced pale-pink colonies, was isolated from tap water on R2A agar at 20 degrees C. 16S rRNA gene sequence studies showed that the strain clustered with Arcicella aquatica (99.0 % sequence similarity to the type strain). The main polyamine present was spermidine, the quinone system comprised menaquinone MK-7 and the polar lipid profile consisted of a mixture of aminophospholipids, aminolipids, glycolipids and uncharacterized polar lipids. The fatty acid profile was slightly different from that reported for A. aquatica. The results of DNA-DNA hybridizations and physiological and biochemical tests clearly allowed both genotypic and phenotypic differentiation of the isolate from A. aquatica. On the basis of these results, it is proposed that the strain should be classified within a novel species of the genus Arcicella. The name proposed for this taxon is Arcicella rosea sp. nov.; the type strain is TW5(T) (=CCUG 55942(T) =CCM 7523(T)).
菌株TW5(T)是一种运动性革兰氏阴性菌，在20℃下于R2A琼脂平板上从自来水中分离得到，能产生浅粉色菌落。16S rRNA基因序列研究表明，该菌株与水生阿氏菌聚为一簇（与模式菌株的序列相似性为99.0%）。主要的多胺为亚精胺，醌系统由甲基萘醌MK-7组成，极性脂质谱由氨基磷脂、氨基脂质、糖脂和未鉴定的极性脂质混合而成。脂肪酸谱与报道的水生阿氏菌略有不同。DNA-DNA杂交以及生理生化试验结果清楚地表明该分离株在基因型和表型上与水生阿氏菌存在差异。基于这些结果，建议将该菌株归为阿氏菌属的一个新物种。为此分类单元提议的名称为玫瑰色阿氏菌（Arcicella rosea sp. nov.）；模式菌株为TW5(T)（=CCUG 55942(T)=CCM 7523(T)）。
