Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, 142 Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):134-140. doi: 10.1099/ijs.0.037242-0. Epub 2012 Feb 24.
A bacterial strain, designated NSW-5(T), was isolated from a water sample taken from Niao-Song Wetland Park in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain NSW-5(T) were strictly aerobic, Gram-stain-negative, non-motile and polymorphic, being straight, vibrioid, curved and spiral-shaped rods surrounded by a thick capsule and forming light pink-coloured colonies. Some rings consisting of several cells were present. Growth occurred at 10-40 °C (optimum, 25 °C), with 0-3.0 % NaCl (optimum, 0 %) and at pH 6.0-8.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NSW-5(T) belonged to the genus Arcicella with sequence similarities of 98.6, 98.0 and 97.3 % with Arcicella aquatica NO-502(T), Arcicella rosea TW5(T) and Arcicella aurantiaca TNR-18(T), respectively. The predominant cellular fatty acids were summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c; 20.8 %), C(16 : 0) (14.6 %), iso-C(15 : 0) (13.8 %), C(16 : 1)ω5c (12.5 %) and C(18 : 0) (11.4 %), and the only respiratory quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized glycolipids, aminolipids, phospholipids and aminophospholipids. The DNA G+C content of strain NSW-5(T) was 44.1 mol%. The DNA-DNA relatedness of strain NSW-5(T) with respect to recognized species of the genus Arcicella was less than 70 %. On the basis of phylogenetic inference and phenotypic data, strain NSW-5(T) should be classified as a representative of a novel species, for which the name Arcicella rigui sp. nov. is proposed. The type strain is NSW-5(T) (= KCTC 23307(T) = BCRC 80260(T)). Emended descriptions of the genus Arcicella and of Arcicella aquatica, Arcicella rosea and Arcicella aurantiaca are also proposed.
一株细菌菌株，命名为 NSW-5(T)，从台湾鸟松湿地公园的水样中分离得到，并通过多相分类学方法进行了表征。菌株 NSW-5(T)的细胞是严格需氧的，革兰氏阴性，不运动，多形性，为直的、弧菌状、弯曲和螺旋形杆状，周围有厚荚膜，形成浅粉色的菌落。存在一些由几个细胞组成的环。生长在 10-40°C（最佳，25°C），0-3.0%NaCl（最佳，0%）和 pH6.0-8.0（最佳，pH7.0）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 NSW-5(T)属于 Arcicella 属，与 Aquatica NO-502(T)、Rosea TW5(T)和 Aurantiaca TNR-18(T)的序列相似性分别为 98.6%、98.0%和 97.3%。主要的细胞脂肪酸为总和特征 3（C(16:1)ω7c 和/或 C(16:1)ω6c；20.8%）、C(16:0)（14.6%）、异-C(15:0)（13.8%）、C(16:1)ω5c（12.5%）和 C(18:0)（11.4%），唯一的呼吸醌是 MK-7。极性脂谱由磷脂酰乙醇胺和几种未鉴定的糖脂、胺脂、磷脂和胺磷脂组成。菌株 NSW-5(T)的 DNA G+C 含量为 44.1mol%。菌株 NSW-5(T)与 Arcicella 属中已识别种的 DNA-DNA 相关性小于 70%。基于系统发育推断和表型数据，菌株 NSW-5(T)应归类为一种新型物种的代表，建议将其命名为 Arcicella rigui sp. nov.。模式菌株为 NSW-5(T)=KCTC 23307(T)=BCRC 80260(T)。还提出了对 Arcicella 属和 Arcicella aquatica、Arcicella rosea 和 Arcicella aurantiaca 的修订描述。
