Cetin Barbaros, Kang Yuejun, Wu Zhemin, Li Dongqing
Department of Mechanical Engineering, Vanderbilt University, Nashville, TN 37235-1592, USA.
Electrophoresis. 2009 Mar;30(5):766-72. doi: 10.1002/elps.200800464.
In this paper, we present a novel, simple lab-on-a-chip device for continuous separation of particles by size. The device is composed of a straight rectangular microchannel connecting two inlet reservoirs and two exit reservoirs. Two asymmetric, 3-D electrodes are embedded along the channel wall to generate a non-uniform electrical field for dielectrophoresis. Particles with different sizes are collected at the different exit reservoirs. Main flow is induced by pressure difference between the inlet and the exit reservoirs. The device is used successfully for the separation of the 5 and 10 mum latex particles and for the separation of yeast cells and white blood cells. A numerical simulation based on Lagrangian tracking method is used to simulate the particle motion and the results showed a good agreement with the experimental data.
在本文中，我们展示了一种新颖、简单的芯片实验室设备，用于按尺寸连续分离颗粒。该设备由连接两个入口储液器和两个出口储液器的直矩形微通道组成。两个不对称的三维电极沿通道壁嵌入，以产生用于介电泳的非均匀电场。不同尺寸的颗粒在不同的出口储液器中收集。主流是由入口和出口储液器之间的压差引起的。该设备已成功用于分离5微米和10微米的乳胶颗粒，以及分离酵母细胞和白细胞。基于拉格朗日跟踪方法的数值模拟用于模拟颗粒运动，结果与实验数据显示出良好的一致性。
