Grinchuk T M, Ivantsov K M, Aleklseenko L L, Kozhukharova I V, Zaĭchik A M, Petrov N S, Mikhaĭlov V M, Popov B V
Tsitologiia. 2008;50(12):1030-5.
We have developed and characterized a murine mesenchymal stem cell line from the bone marrow of transgenic mouse C57BL ubiquitously expressing GFP. Immunostaining analysis revealed the presence of several markers typically found in fibroblasts such as smooth muscle cells actin in the form of stress fibrils and vimentin--the protein of intermediate filaments. These cells maintained capability to differentiate into adipocytes or osteoblasts under appropriate conditions. Karyotypic features include changes in the ploidy level between 2n and 8n and multiple chromosomal aberrations. After six passages 80% of the cell population was aneuploid with chromosomal numbers between 50 and 85 without well defined modal class. Differential G-staining of metaphase spreads showed variability in copy numbers of individual chromosomes and the presence of aberrations such as ectopic associations of non-homologous chromosomes. All analyzed cells contained unique dicentric marker chromosome and some of them also had numerous microchromosomes which might indicate gene amplification. These cells could be useful in the future work directed at the investigation of stem cells spontaneous oncogenic transformation in vivo and in vitro.
我们从普遍表达绿色荧光蛋白（GFP）的转基因小鼠C57BL的骨髓中开发并鉴定了一种小鼠间充质干细胞系。免疫染色分析显示存在几种通常在成纤维细胞中发现的标志物，如以应力纤维形式存在的平滑肌细胞肌动蛋白和波形蛋白——中间丝蛋白。这些细胞在适当条件下保持分化为脂肪细胞或成骨细胞的能力。核型特征包括2n至8n倍体水平的变化以及多个染色体畸变。传代六次后，80%的细胞群体为非整倍体，染色体数在50至85之间，无明确的众数类。中期染色体铺展的差异G染色显示个别染色体拷贝数的变异性以及非同源染色体异位关联等畸变的存在。所有分析的细胞都含有独特的双着丝粒标记染色体，其中一些还具有许多微染色体，这可能表明基因扩增。这些细胞在未来旨在研究干细胞体内外自发致癌转化的工作中可能会有用。
