Strable E, Finn M G
Dynavax Technologies Corp., Berkeley, CA 94710-2753, USA.
Curr Top Microbiol Immunol. 2009;327:1-21. doi: 10.1007/978-3-540-69379-6_1.
Protein capsids derived from viruses may be modified by methods, generated, isolated, and purified on large scales with relative ease. In recent years, methods for their chemical derivatization have been employed to broaden the properties and functions accessible to investigators desiring monodisperse, atomic-resolution structures on the nanometer scale. Here we review the reactions and methods used in these endeavors, including the modification of lysine, cysteine, and tyrosine side chains, as well as the installation of unnatural amino acids, with particular attention to the special challenges imposed by the polyvalency and size of virus-based scaffolds.
源自病毒的蛋白质衣壳可以通过相对容易大规模生成、分离和纯化的方法进行修饰。近年来，已采用其化学衍生化方法来拓展纳米尺度下单分散、原子分辨率结构的研究者可利用的性质和功能。在此，我们综述这些研究中所使用的反应和方法，包括赖氨酸、半胱氨酸和酪氨酸侧链的修饰，以及非天然氨基酸的引入，并特别关注基于病毒的支架的多价性和尺寸所带来的特殊挑战。
