Bina David, Litvin Radek, Vacha Frantisek
Biology Centre of AVCR, v.v.i, Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic.
Photosynth Res. 2009 Feb;99(2):115-25. doi: 10.1007/s11120-009-9408-4. Epub 2009 Feb 6.
The light-induced electron transport in purple bacterium Rhodobacter sphaeroides was studied in vivo by means of kinetic difference absorption spectroscopy and kinetics of bacteriochlorophyll fluorescence yield. Measurements of redox state of the oxidised primary donor and cytochrome c and the membrane potential revealed a complex pattern of changes of the electron flow. Effects of the membrane potential on the fluorescence yield were also analysed, and a model for the fluorescence induction curve is presented. The data indicate substantial positive effect of the membrane potential on the fluorescence emission in vivo. Moreover, light-induced changes in light scattering were observed, which suggests occurrence of structural changes on the level of the photosynthetic membrane.
通过动力学差示吸收光谱法和细菌叶绿素荧光产率动力学，在体内研究了球形红杆菌中的光诱导电子传递。对氧化态初级供体和细胞色素c的氧化还原状态以及膜电位的测量揭示了电子流变化的复杂模式。还分析了膜电位对荧光产率的影响，并提出了荧光诱导曲线的模型。数据表明膜电位对体内荧光发射有显著的正向作用。此外，观察到光诱导的光散射变化，这表明在光合膜水平上发生了结构变化。
