State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, No 73 Huanghe Road, 2nd Campus of HIT Box 2614, Harbin 150090, China.
Bioresour Technol. 2010 Jul;101(14):5325-9. doi: 10.1016/j.biortech.2010.02.024. Epub 2010 Mar 4.
In this study, some key factors, for example, diluted ratio of effluents, the ratio of dark-photo bacteria, light intensity and light/dark cycle influencing hydrogen production by combining Clostridium butyricum and immobilized Rhodopseudomonas faecalis RLD-53 in batch culture, were investigated. Experimental results showed the photo-hydrogen yield decreased when increasing diluted ratio from 1:0.5 to 1:3, and it reached the maximum value of 4368 ml-H(2)/l-effluents at the ratio of 1:0.5. When the ratio of dark-photo bacteria was at 1:2, the hydrogen yield reached highest value of 4.946 mol-H(2)/mol-glucose and cumulative hydrogen volume was 5357 ml-H(2)/l-culture during the combination process. When the light intensity was at 10.25 W/m(2), the hydrogen volume of photo-fermentation and the combination process reached maximum value of 4260 ml-H(2)/l-effluents and 5892 ml-H(2)/l-culture, respectively. During the combination process, maximum total hydrogen yield was 5.374 mol-H(2)/mol-glucose. Meanwhile, hydrogen production under light/dark cycle was evaluated.
在这项研究中，考察了一些关键因素，例如在批式培养中结合丁酸梭菌和固定化荚膜红假单胞菌 RLD-53 来影响产氢时，出水的稀释比、暗-光细菌比、光强和光/暗循环的影响。实验结果表明，随着稀释比从 1:0.5 增加到 1:3，光产氢率降低，在 1:0.5 的比例下达到 4368 ml-H2/ l-废水的最大值。当暗-光细菌比为 1:2 时，在组合过程中，产氢量达到 4.946 mol-H2/mol-葡萄糖的最高值，累积氢气体积为 5357 ml-H2/ l-培养物。当光强为 10.25 W/m2 时，光发酵和组合过程的氢气体积分别达到 4260 ml-H2/ l-废水和 5892 ml-H2/ l-培养物的最大值。在组合过程中，最大总产氢量为 5.374 mol-H2/mol-葡萄糖。同时，评估了光/暗循环下的产氢情况。
