在批式培养中通过暗发酵和光发酵联合实现生物制氢的强化。
Enhanced bio-hydrogen production by the combination of dark- and photo-fermentation in batch culture.
机构信息
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, No 73 Huanghe Road, 2nd Campus of HIT Box 2614, Harbin 150090, China.
出版信息
Bioresour Technol. 2010 Jul;101(14):5325-9. doi: 10.1016/j.biortech.2010.02.024. Epub 2010 Mar 4.
In this study, some key factors, for example, diluted ratio of effluents, the ratio of dark-photo bacteria, light intensity and light/dark cycle influencing hydrogen production by combining Clostridium butyricum and immobilized Rhodopseudomonas faecalis RLD-53 in batch culture, were investigated. Experimental results showed the photo-hydrogen yield decreased when increasing diluted ratio from 1:0.5 to 1:3, and it reached the maximum value of 4368 ml-H(2)/l-effluents at the ratio of 1:0.5. When the ratio of dark-photo bacteria was at 1:2, the hydrogen yield reached highest value of 4.946 mol-H(2)/mol-glucose and cumulative hydrogen volume was 5357 ml-H(2)/l-culture during the combination process. When the light intensity was at 10.25 W/m(2), the hydrogen volume of photo-fermentation and the combination process reached maximum value of 4260 ml-H(2)/l-effluents and 5892 ml-H(2)/l-culture, respectively. During the combination process, maximum total hydrogen yield was 5.374 mol-H(2)/mol-glucose. Meanwhile, hydrogen production under light/dark cycle was evaluated.
在这项研究中，考察了一些关键因素，例如在批式培养中结合丁酸梭菌和固定化荚膜红假单胞菌 RLD-53 来影响产氢时，出水的稀释比、暗-光细菌比、光强和光/暗循环的影响。实验结果表明，随着稀释比从 1:0.5 增加到 1:3，光产氢率降低，在 1:0.5 的比例下达到 4368 ml-H2/ l-废水的最大值。当暗-光细菌比为 1:2 时，在组合过程中，产氢量达到 4.946 mol-H2/mol-葡萄糖的最高值，累积氢气体积为 5357 ml-H2/ l-培养物。当光强为 10.25 W/m2 时，光发酵和组合过程的氢气体积分别达到 4260 ml-H2/ l-废水和 5892 ml-H2/ l-培养物的最大值。在组合过程中，最大总产氢量为 5.374 mol-H2/mol-葡萄糖。同时，评估了光/暗循环下的产氢情况。
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