天然产物生物合成中的酶工程机遇。
Opportunities for enzyme engineering in natural product biosynthesis.
作者信息
Bernhardt Peter, O'Connor Sarah E
机构信息
Massachusetts Institute of Technology, Department of Chemistry, Cambridge, 02139, USA.
出版信息
Curr Opin Chem Biol. 2009 Feb;13(1):35-42. doi: 10.1016/j.cbpa.2009.01.005. Epub 2009 Feb 5.
Abstract
Organisms from all kingdoms of life produce a plethora of natural products that display a range of biological activities. One key limitation of developing these natural products into pharmaceuticals is the inability to perform effective, fast, and inexpensive structure-activity relationship studies (SAR). Recently, enzyme engineering strategies have allowed the exploration of metabolic engineering of biosynthetic pathways to create new 'natural' products that can be used for SAR. The enzymes that enable the biosynthesis of natural products represent a largely untapped resource of potential biocatalysts. A challenge for the field is how to harness the wealth of reaction types used for natural product metabolism to obtain useful biocatalysts for industrial biotransformations.
摘要
来自生命所有王国的生物体都会产生大量具有一系列生物活性的天然产物。将这些天然产物开发成药物的一个关键限制是无法进行有效、快速且廉价的构效关系研究（SAR）。最近，酶工程策略使得人们能够探索生物合成途径的代谢工程，以创造可用于构效关系研究的新“天然”产物。能够实现天然产物生物合成的酶代表了一个基本上未被开发的潜在生物催化剂资源。该领域面临的一个挑战是如何利用用于天然产物代谢的丰富反应类型来获得用于工业生物转化的有用生物催化剂。
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