蛋白质工程在提高和多样化天然产物生物合成中的应用。
Protein Engineering for Improving and Diversifying Natural Product Biosynthesis.
机构信息
School of Chemical, Materials, and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA 30602, USA.
School of Chemical, Materials, and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA 30602, USA.
出版信息
Trends Biotechnol. 2020 Jul;38(7):729-744. doi: 10.1016/j.tibtech.2019.12.008. Epub 2020 Jan 15.
Abstract
Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosynthesis of natural products. Recently, protein engineering has become a favored method to improve enzymatic activity, increase enzyme stability, and expand product spectra in natural product biosynthesis. This review summarizes recent advances and typical strategies in protein engineering, highlighting the paramount role of protein engineering in improving and diversifying the biosynthesis of natural products. Future prospects and research directions are also discussed.
摘要
天然存在的蛋白质一直是最常用的生物催化剂，用于生产从大宗商品化学品到药物的各种天然产物。蛋白质工程已成为代谢工程开发的有力生物技术工具箱，特别是在天然产物的生物合成方面。最近，蛋白质工程已成为提高酶活性、增加酶稳定性和扩大天然产物生物合成产物谱的首选方法。本文综述了蛋白质工程的最新进展和典型策略，强调了蛋白质工程在改善和多样化天然产物生物合成方面的重要作用。还讨论了未来的前景和研究方向。
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