Dogru Salim, Haholu Abdulah, Gungor Atila, Kucukodaci Zafer, Cincik Hakan, Ozdemir Taner, Sen Huseyin
Department of Otolaryngology, Haydarpasa Training Hospital, Istanbul, Turkey.
Otolaryngol Head Neck Surg. 2009 Feb;140(2):177-82. doi: 10.1016/j.otohns.2008.10.023.
To investigate histologic changes in the mucosa of rat middle ear after implantation of three different support materials.
A prospective, controlled animal study.
Three types of absorbable materials were implanted into the middle ear cavity of rats: (1) Gelfoam (purified gelatin) (Pharmacia & Upjohn Company, New York, NY), (2) Sepragel (viscoelastic gel composed of cross-linked polymers of hyaluronan) (GENZYME Corp, Ridgefield, NJ), and (3) Nasopore (a biodegradable/fragmentable, synthetic polyurethane foam) (Polyganics, Groningen, The Netherlands). Rats were sacrificed after 3 and 20 days to ascertain early and late histologic changes. The bulla of each rat was excised and prepared for microscopic examination. The histologic changes were evaluated by observation of the middle ear cavity and mucosa in terms of polymorphonuclear leucocytes (PMNL), macrophages, giant cells, fibroblasts and other cells, fibrosis, and remnant materials.
The histologic appearance of gelfoam-treated middle ears was characterized by more severe acute inflammation in the short-term and prominent fibrosis in the long-term in comparison with sepragel- and nasopore-treated groups. Nasopore appeared to be prone to remnant formation and reorganization by means of fibroblastic activity.
Compared with gelfoam, both sepragel and nasopore caused less histologic alterations.
研究三种不同支撑材料植入大鼠中耳后黏膜的组织学变化。
一项前瞻性对照动物研究。
将三种可吸收材料植入大鼠中耳腔：（1）明胶海绵（纯化明胶）（法玛西亚普强公司，纽约州纽约市），（2）Sepragel（由透明质酸交联聚合物组成的粘弹性凝胶）（健赞公司，新泽西州里奇菲尔德），以及（3）鼻用海绵（一种可生物降解/可破碎的合成聚氨酯泡沫）（Polyganics公司，荷兰格罗宁根）。在3天和20天后处死大鼠，以确定早期和晚期组织学变化。切除每只大鼠的鼓泡并制备用于显微镜检查。通过观察中耳腔和黏膜中的多形核白细胞（PMNL）、巨噬细胞、巨细胞、成纤维细胞和其他细胞、纤维化以及残留材料来评估组织学变化。
与Sepragel和鼻用海绵治疗组相比，明胶海绵治疗的中耳组织学表现为短期更严重的急性炎症和长期明显的纤维化。鼻用海绵似乎易于通过成纤维细胞活性形成残留并进行重塑。
与明胶海绵相比，Sepragel和鼻用海绵引起的组织学改变较少。
