Jacobelli Jordan, Bennett F Chris, Pandurangi Priya, Tooley Aaron J, Krummel Matthew F
Department of Pathology, University of California San Francisco, San Francisco CA 94143, USA.
J Immunol. 2009 Feb 15;182(4):2041-50. doi: 10.4049/jimmunol.0803267.
How T cells achieve rapid chemotactic motility under certain circumstances and efficient cell surface surveillance in others is not fully understood. We show that T lymphocytes are motile in two distinct modes: a fast "amoeboid-like" mode, which uses sequential discontinuous contacts to the substrate; and a slower mode using a single continuously translating adhesion, similar to mesenchymal motility. Myosin-IIA is necessary for fast amoeboid motility, and our data suggests that this occurs via cyclical rear-mediated compressions that eliminate existing adhesions while licensing subsequent ones at the front of the cell. Regulation of Myosin-IIA function in T cells is thus a key mechanism to regulate surface contact area and crawling velocity within different environments. This can provide T lymphocytes with motile and adhesive properties that are uniquely suited toward alternative requirements for immune surveillance and response.
T细胞如何在某些情况下实现快速趋化运动，而在其他情况下实现高效的细胞表面监测，目前尚未完全了解。我们发现T淋巴细胞以两种不同模式运动：一种快速的“类阿米巴样”模式，该模式利用与底物的连续间断接触；另一种较慢的模式，利用单一持续移动的黏附，类似于间充质运动。肌球蛋白-IIA是快速类阿米巴样运动所必需的，我们的数据表明，这是通过周期性的后部介导压缩发生的，这种压缩消除了现有的黏附，同时允许在细胞前端形成后续黏附。因此，T细胞中肌球蛋白-IIA功能的调节是调节不同环境中表面接触面积和爬行速度的关键机制。这可以为T淋巴细胞提供独特适合免疫监测和反应的替代需求的运动和黏附特性。
