Tanimoto Shinichi, Miyazawa Mitsuo, Inoue Toshio, Okada Yoshiharu, Nomura Masato
Graduate School of System Engineering, Program in Systems Engineering, Kinki University, 1 Umenobe, Takaya, Higashihiroshima, Hiroshima 739-2116, Japan.
J Oleo Sci. 2009;58(3):141-6. doi: 10.5650/jos.58.141.
Petals from Coreopsis lanceolata L. were extracted in methanol and subjected to solvent-solvent partitioning to yield four fractions: hexane, diethyl ether, ethyl acetate, and aqueous. These fractions were screened using a 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay and an active oxygen inhibition assay. The ethyl acetate extract contained the highest level of active components. Four compounds isolated from this fraction by HPLC were screened for antioxidant activity and anti-allergic activity. Anti-allergic activity was assessed by measuring release of histamine from rat peritoneal mast cells. One compound, termed laceolin, was characterized by spectroscopic analysis, including extensive NMR and mass spectra. This compound had significant radical scavenging activity, with an SC50 (scavenging concentration of 50%) value of 2.6 microg/mL and superoxide dismutase activity of 46.2%.
将柳叶金鸡菊的花瓣用甲醇提取，然后进行溶剂-溶剂分配，得到四个馏分：己烷、二乙醚、乙酸乙酯和水相。使用1,1-二苯基-2-苦基肼自由基清除试验和活性氧抑制试验对这些馏分进行筛选。乙酸乙酯提取物中活性成分含量最高。通过高效液相色谱从该馏分中分离出的四种化合物进行了抗氧化活性和抗过敏活性筛选。通过测量大鼠腹膜肥大细胞组胺释放来评估抗过敏活性。一种名为laceolin的化合物通过光谱分析进行了表征，包括广泛的核磁共振和质谱。该化合物具有显著的自由基清除活性，SC50（清除浓度为50%）值为2.6微克/毫升，超氧化物歧化酶活性为46.2%。
