Bounamous A, Boudabous R, Jouet D, Augot D, Ferté H, Babba H, Berchi S, Depaquit J
JE 2533-USC AFSSA "VECPAR", Faculté de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.
Parasite. 2008 Dec;15(4):565-71. doi: 10.1051/parasite/2008154565.
In 1998, the description of Phlebotomus riouxi emphasised the difficulty to differentiate the female from the closely related P. chabaudi, a suspected vector of Leishmania killicki in several foci in Tunisia. In order to be able to distinguish the females of these two species, we started a molecular study based on 37 Algerian and Tunisian specimens. The alignment of the sequences of the cytochrome b of the mitochondrial DNA and their analysis using Neighbor-Joining, maximum likelihood and maximum parsimony shows the individualisation of two species including an intraspecific variability. Following a morphological approach, it is not possible to distinguish the females on the basis of their spermathecae. A new character is proposed: the presence of anterolateral teeth of the pharyngeal armature for P. chabaudi, never observed in P. riouxi. However, a molecular typing is necessary at the present time for a sure identification of the females.
1998年，关于里乌西白蛉的描述强调了将雌虫与亲缘关系密切的沙氏白蛉区分开来的困难，沙氏白蛉在突尼斯的几个疫源地被怀疑是基氏利什曼原虫的传播媒介。为了能够区分这两个物种的雌虫，我们基于37个阿尔及利亚和突尼斯标本开展了一项分子研究。线粒体DNA细胞色素b序列的比对以及使用邻接法、最大似然法和最大简约法对其进行的分析显示了两个物种的个体化，包括种内变异性。采用形态学方法，无法根据受精囊区分雌虫。提出了一个新特征：沙氏白蛉咽骨有前侧齿，而在里乌西白蛉中从未观察到。然而，目前为了准确鉴定雌虫，分子分型是必要的。
