Batista Ivani Aparecida Soares de Andrade, Gonçalves Maria Inês de Almeida, Singh Anil Kumar, Hackmann Erika Rosa Maria Kedor, Santoro Maria Inês Rocha Miritello
University of São Paulo, Department of Pharmacy, Faculty of Pharmaceutical Sciences, CEP 05508-000, São Paulo-SP, Brazil.
J AOAC Int. 2008 Nov-Dec;91(6):1303-8.
A nuclear magnetic resonance (NMR) spectroscopic method was validated for the quantitative determination of dimethylaminoethanol (DMAE) in cosmetic formulations. The linearity in the range from 0.5000 to 1.5000 g (DMAE salt/mass maleic acid) presents a correlation coefficient > 0.99 for all DMAE salts. The repeatability (intraday), expressed as relative standard deviation, ranged from 1.08 to 1.44% for samples and 1.31 to 1.88% for raw materials. The detection limit and quantitation limit were 0.0017 and 0.0051 g for DMAE, 0.0018 and 0.0054 g for DMAE bitartrate, and 0.0023 and 0.0071 g for DMAE acetamidobenzoate, respectively. The proposed method is simple, precise, and accurate and can be used in the quality control of raw materials and cosmetic gels containing these compounds as active substances.
一种核磁共振（NMR）光谱法经过验证，可用于定量测定化妆品配方中的二甲基氨基乙醇（DMAE）。对于所有DMAE盐，在0.5000至1.5000 g（DMAE盐/马来酸质量）范围内的线性关系呈现出相关系数>0.99。以相对标准偏差表示的重复性（日内），样品范围为1.08%至1.44%，原料范围为1.31%至1.88%。DMAE的检测限和定量限分别为0.0017 g和0.0051 g，酒石酸氢盐DMAE分别为0.0018 g和0.0054 g，乙酰氨基苯甲酸盐DMAE分别为0.0023 g和0.0071 g。所提出的方法简单、精确且准确，可用于含有这些化合物作为活性物质的原料和化妆品凝胶的质量控制。
