Lee V D, Stapleton M, Huang B
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
J Mol Biol. 1991 Sep 5;221(1):175-91.
A clone containing the gene locus for Chlamydomonas caltractin, a 20,000 Mr calcium-binding protein that is a member of the EF-hand superfamily of calcium-modulated proteins, was isolated and the structural organization of the gene was determined. The intron-exon organization was resolved by direct comparison of the genomic sequence with a caltractin cDNA. The promoter region does not contain the typical TATA or CCAAT boxes, but the sequences at the splice junctions are similar to those of other eukaryotes. The positions of the six introns in the caltractin gene do not typically define unit structures, nor do they coincide with those in genes for other members of the EF-hand superfamily. An analysis of exon sequences at the splice junctions in the genes of this multigene family was undertaken; evidence was obtained that supports the hypothesis that introns arose at proto-splice sites. A probable evolutionary history for the EF-hand superfamily based on intron insertion is offered.
分离出了一个含有衣藻钙结合蛋白基因位点的克隆，该蛋白分子量为20,000，是钙调节蛋白EF-手超家族的成员，并确定了该基因的结构组织。通过将基因组序列与钙结合蛋白cDNA直接比较，解析了内含子-外显子组织。启动子区域不包含典型的TATA或CCAAT框，但剪接位点的序列与其他真核生物的序列相似。钙结合蛋白基因中六个内含子的位置通常并不定义单位结构，也与EF-手超家族其他成员基因中的内含子位置不一致。对这个多基因家族基因剪接位点的外显子序列进行了分析；获得的证据支持内含子起源于原剪接位点的假说。基于内含子插入，提出了EF-手超家族可能的进化史。
