亲水性磁性纳米颗粒在生物相关条件下的稳定性。

Stability of hydrophilic magnetic nanoparticles under biologically relevant conditions.

作者信息

Chen Z P, Zhang Y, Xu K, Xu R Z, Liu J W, Gu N

机构信息

Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.

出版信息

J Nanosci Nanotechnol. 2008 Dec;8(12):6260-5.

PMID:19205192

Abstract

Hydrophilic 2,3-dimercaptosuccinnic acid (HOOC-CH(SH)-CH(SH)-COOH, DMSA) coated monodisperse magnetic nanoparticles (Fe3O4) were dispersed in water, RPMI-1640 with 10% (v/v) fetal calf serum, RPMI-1640, PBS and MES, respectively, to investigate their stability under biologically relevant conditions. The Photon Correlation Spectroscopy (PCS) results showed that DMSA-Fe3O4 nanoparticles existed as aggregate under biological conditions. UV-vis, MRI and AFM results indicated that DMSA-Fe3O4 nanoparticles dispersed in RPMI-1640, PBS and MES presented poor stability, whereas those dispersed in RPMI-1640 with fetal calf serum exhibited excellent stability, which was due to their adsorption from fetal calf serum, as confirmed by zeta potential and IR results. Additionally, in vitro cell experiments showed that DMSA-Fe3O4 nanoparticles with adsorption from fetal calf serum had higher intracellular uptake than those without adsorption from serum, indicating that fetal calf serum could play a great role in intracellular uptake of nanoparticles.

摘要

将亲水性的2,3-二巯基琥珀酸（HOOC-CH(SH)-CH(SH)-COOH，DMSA）包覆的单分散磁性纳米颗粒（Fe3O4）分别分散于水、含10%（v/v）胎牛血清的RPMI-1640培养基、RPMI-1640培养基、磷酸盐缓冲液（PBS）和2-(N-吗啉代)乙磺酸（MES）中，以研究其在生物学相关条件下的稳定性。光子相关光谱（PCS）结果表明，DMSA-Fe3O4纳米颗粒在生物学条件下以聚集体形式存在。紫外可见光谱、磁共振成像（MRI）和原子力显微镜（AFM）结果表明，分散于RPMI-1640培养基、PBS和MES中的DMSA-Fe3O4纳米颗粒稳定性较差，而分散于含胎牛血清的RPMI-1640培养基中的纳米颗粒表现出优异的稳定性，这是由于它们从胎牛血清中吸附，ζ电位和红外光谱结果证实了这一点。此外，体外细胞实验表明，吸附了胎牛血清的DMSA-Fe3O4纳米颗粒比未吸附血清的纳米颗粒具有更高的细胞内摄取率，表明胎牛血清在纳米颗粒的细胞内摄取中可发挥重要作用。