Wesley Irene V, Rostagno Marcos, Hurd H Scott, Trampel Darrell W
Pre-Harvest Food Safety and Enteric Diseases Unit, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, Ames, Iowa 50010, USA.
J Food Prot. 2009 Jan;72(1):43-8. doi: 10.4315/0362-028x-72.1.43.
To monitor the effects of feed withdrawal on the prevalence of Campylobacter, market-weight turkeys from six farms were examined before and after perimarketing events (feed withdrawal, transport, and holding at the slaughterhouse). Prior to transport, birds (n = 30 per farm) were slaughtered on-farm, and viscera (crops, duodenum, jejunum, ileum, colon, ceca, gallbladder, and spleen) were removed on the premises. Within ca. 48 h, cohorts (n = 30 per farm) from the same flock were transported to a commercial abattoir, maintained in holding sheds, slaughtered, and the viscera were removed. No differences in the prevalence of Campylobacter spp. were evident when individual flocks were compared pre- and posttransport. However, when data for the six farms were combined, Campylobacter spp. were recovered (pre- versus posttransport) at comparable rates from the duodenum (74.7 versus 74.7%), ileum (87.3 versus 92.7%), ceca (64 versus 57%), colon (86.7 versus 80%), and spleen (0 versus 0%). After feed withdrawal, transport, and holding at the abattoir, there was an overall increase in Campylobacter spp. isolated from the gallbladder at the abattoir (14.7%) when compared with on-farm levels (0%, P < 0.05). When compared with on-farm levels (3%), the overall increase in Campylobacter spp. recovered from the crops of birds at the abattoir (24%) was significant (P < 0.05), which may be associated with a detectable decline in lactic acid in the emptied crop.
为监测停喂饲料对弯曲杆菌流行率的影响，在六个农场的上市体重火鸡进行售前相关活动（停喂饲料、运输以及在屠宰场暂存）前后对其进行了检查。运输前，在农场宰杀禽类（每个农场30只），并在现场取出内脏（嗉囊、十二指肠、空肠、回肠、结肠、盲肠、胆囊和脾脏）。在约48小时内，将来自同一鸡群的一组禽类（每个农场30只）运输至商业屠宰场，在暂存棚中饲养，然后宰杀并取出内脏。比较各个鸡群运输前后弯曲杆菌属的流行率时，未发现明显差异。然而，将六个农场的数据合并后，从十二指肠（74.7%对74.7%）、回肠（87.3%对92.7%）、盲肠（64%对57%）、结肠（86.7%对80%）和脾脏（0对0）中回收弯曲杆菌属的比例在运输前后相当。在停喂饲料、运输以及在屠宰场暂存后，与农场水平相比，从屠宰场禽类胆囊中分离出的弯曲杆菌属总体有所增加（14.7%，而农场水平为0%，P<0.05）。与农场水平（3%）相比，从屠宰场禽类嗉囊中回收的弯曲杆菌属总体增加（24%）具有显著性（P<0.05），这可能与排空嗉囊中乳酸的可检测下降有关。
