Yu Zheng, Haage Kristina, Streit Verena E, Gierl Alfons, Ruiz Ramón A Torres
Lehrstuhl für Genetik, Wissenschaftszentrum Weihenstephan, Technische Universität München, Am Hochanger 8, 85350, Freising, Germany.
Theor Appl Genet. 2009 Apr;118(6):1107-19. doi: 10.1007/s00122-009-0966-9. Epub 2009 Feb 11.
Arabidopsis thaliana has, in conjunction with A. arenosa, developed into a system for the molecular analysis of alloplolyploidy. However, there are very few Arabidopsis lines available to study autopolyploidy. In order to investigate polyploidy on a reliable basis, we have optimised conventional methodologies and developed a novel strategy for the rapid generation and identification of polyploids based on trichome branching patterns. The analysis of more than two dozen independently induced Arabidopsis lines has led to interesting observations concerning the relationship between cell size and ploidy levels and on the relative stability of tetraploidy in Arabidopsis over at least three consecutive generations. The most important finding of this work is that neo-tetraploid lines exhibit considerable stability through all the generations tested. The systematic generation of tetraploid collections through this strategy as well as the lines generated in this work will help to unravel the consequences of polyploidy, particularly tetraploidy, on the genome, on gene expression and on natural diversity in Arabidopsis.
拟南芥与砂生拟南芥一起，已发展成为一个用于异源多倍体分子分析的系统。然而，可用于研究同源多倍体的拟南芥品系非常少。为了在可靠的基础上研究多倍体，我们优化了传统方法，并基于毛状体分支模式开发了一种快速产生和鉴定多倍体的新策略。对二十多个独立诱导的拟南芥品系的分析得出了一些有趣的观察结果，这些结果涉及细胞大小与倍性水平之间的关系，以及拟南芥四倍体在至少连续三代中的相对稳定性。这项工作最重要的发现是，新四倍体系在所有测试代中都表现出相当的稳定性。通过这种策略系统地产生四倍体集合以及在这项工作中产生的品系，将有助于揭示多倍体，特别是四倍体，对拟南芥基因组、基因表达和自然多样性的影响。
