富血小板血浆局部给药后髌腱缺损血管生成的免疫组织化学研究。
Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect.
机构信息
Orthopaedic Clinic, Democritus University of Thrace, Alexandroupolis, Greece.
出版信息
Int Orthop. 2010 Feb;34(1):143-8. doi: 10.1007/s00264-009-0728-y. Epub 2009 Feb 11.
Abstract
A full thickness defect was made in the central portion of the patellar tendon of 48 New Zealand white rabbits. Platelet-rich plasma (PRP) gel was then applied and filled the tendon defect. The same procedure was performed in the control group, without the application of PRP. Animals were sacrificed after one, two, three, and four weeks. Histological and immunohistochemical analyses using a monoclonal antibody against CD31 were performed. The histological examination showed a superior healing process in the PRP group compared with the control group. Especially in the third week, the tissue formed in the PRP group was more mature and dense with less elastic fibres remaining. Neovascularisation was significantly higher in the PRP group during the first two weeks and significantly lower in the third and fourth weeks (p < 0.0001). Histological examination and study of angiogenesis showed that the application of PRP enhances and accelerates the tendon healing process.
摘要
在 48 只新西兰白兔的髌腱中央部位制造全层缺损。然后应用富含血小板的血浆（PRP）凝胶并填充腱缺损。在对照组中进行了相同的程序，而没有应用 PRP。动物在 1、2、3 和 4 周后被处死。使用针对 CD31 的单克隆抗体进行组织学和免疫组织化学分析。组织学检查显示 PRP 组的愈合过程优于对照组。特别是在第 3 周，PRP 组形成的组织更成熟且更密集，残留的弹性纤维更少。在最初的两周内，PRP 组的新生血管化明显更高，而在第 3 和第 4 周则明显更低（p<0.0001）。组织学检查和血管生成研究表明，PRP 的应用可增强和加速腱愈合过程。
相似文献
1
Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect.富血小板血浆局部给药后髌腱缺损血管生成的免疫组织化学研究。
Int Orthop. 2010 Feb;34(1):143-8. doi: 10.1007/s00264-009-0728-y. Epub 2009 Feb 11.
2
The influence of platelet-rich plasma on angiogenesis during the early phase of tendon healing.富含血小板血浆对肌腱愈合早期血管生成的影响。
Foot Ankle Int. 2009 Nov;30(11):1101-6. doi: 10.3113/FAI.2009.1101.
3
Effect of a single versus serial platelet-rich plasma injection on the healing of acute patellar tendon defect: an experimental study.单次与连续富血小板血浆注射对急性髌腱缺损愈合的影响：一项实验研究。
BMC Musculoskelet Disord. 2024 Aug 30;25(1):684. doi: 10.1186/s12891-024-07804-4.
4
Experimental study of tendon healing early phase: is IGF-1 expression influenced by platelet rich plasma gel?肌腱愈合早期的实验研究：富血小板血浆凝胶是否影响 IGF-1 的表达？
Orthop Traumatol Surg Res. 2010 Jun;96(4):381-7. doi: 10.1016/j.otsr.2010.03.010. Epub 2010 May 7.
5
Comparison of the effects of human recombinant epidermal growth factor and platelet-rich plasma on healing of rabbit patellar tendon.重组人表皮生长因子与富血小板血浆对兔髌腱愈合影响的比较
Eklem Hastalik Cerrahisi. 2017 Aug;28(2):92-9. doi: 10.5606/ehc.2017.55396.
6
The effect of platelet-rich plasma gel in the early phase of patellar tendon healing.富血小板血浆凝胶对髌腱愈合早期的影响。
Arch Orthop Trauma Surg. 2009 Nov;129(11):1577-82. doi: 10.1007/s00402-009-0935-4. Epub 2009 Jul 21.
7
Temporal and Spatial Expression of TGF-b1 in the Early Phase of Patellar Tendon Healing after Application of Platelet Rich Plasma.富含血小板血浆应用后髌腱愈合早期TGF-b1的时空表达
Arch Bone Jt Surg. 2016 Apr;4(2):156-60.
8
Autologous Freeze-Dried, Platelet-Rich Plasma Carrying Icariin Enhances Bone-Tendon Healing in a Rabbit Model.自体冻干富血小板血浆携带淫羊藿苷增强兔模型的骨-腱愈合。
Am J Sports Med. 2019 Jul;47(8):1964-1974. doi: 10.1177/0363546519849657. Epub 2019 May 31.
9
Platelet-rich plasma (PRP) accelerates murine patellar tendon healing through enhancement of angiogenesis and collagen synthesis.富含血小板血浆（PRP）通过促进血管生成和胶原蛋白合成加速小鼠髌腱愈合。
J Exp Orthop. 2020 Jul 8;7(1):49. doi: 10.1186/s40634-020-00267-1.
10
Eccentric Training for Tendon Healing After Acute Lesion: A Rat Model.急性损伤后肌腱愈合的离心训练：大鼠模型
Am J Sports Med. 2017 May;45(6):1440-1446. doi: 10.1177/0363546517689872. Epub 2017 Mar 14.
引用本文的文献
1
The Effects of Tissue Healing Factors in Wound Repair Involving Absorbable Meshes: A Narrative Review.组织愈合因子在可吸收网片伤口修复中的作用：一项叙述性综述
J Clin Med. 2023 Aug 31;12(17):5683. doi: 10.3390/jcm12175683.
2
Injection Treatments for Vulvovaginal Atrophy of Menopause: A Systematic Review.绝经后女性阴道萎缩的注射治疗：系统评价。
Aesthetic Plast Surg. 2023 Dec;47(6):2788-2799. doi: 10.1007/s00266-023-03550-5. Epub 2023 Aug 14.
3
Public Interest in Shoulder Platelet-Rich Plasma Injections Is Increasing: A 10-Year Google Trends Analysis.公众对肩部富血小板血浆注射的兴趣正在增加：一项为期10年的谷歌趋势分析。
Arthrosc Sports Med Rehabil. 2023 Jul 13;5(4):100744. doi: 10.1016/j.asmr.2023.04.026. eCollection 2023 Aug.
4
The effects of orthobiologics in the treatment of tendon pathologies: a systematic review of preclinical evidence.骨科生物制剂在肌腱疾病治疗中的作用：临床前证据的系统评价
J Exp Orthop. 2022 Apr 8;9(1):31. doi: 10.1186/s40634-022-00468-w.
5
Efficacy of Local Autologous Platelet-Rich Plasma in the Treatment of Pressure Ulcer in Spinal Cord Injury Patients.局部自体富血小板血浆治疗脊髓损伤患者压疮的疗效
Cureus. 2021 Oct 11;13(10):e18668. doi: 10.7759/cureus.18668. eCollection 2021 Oct.
6
Needle tenotomy with PRP versus lidocaine in epicondylopathy: clinical and ultrasonographic outcomes over twenty months.针刀松解术联合富血小板血浆与利多卡因治疗肱骨外上髁炎：20 个月的临床和超声结果。
Skeletal Radiol. 2019 Sep;48(9):1399-1409. doi: 10.1007/s00256-019-03193-1. Epub 2019 Mar 2.
7
Current trends in tendinopathy: consensus of the ESSKA basic science committee. Part II: treatment options.肌腱病的当前趋势：欧洲运动医学学会基础科学委员会共识。第二部分：治疗选择。
J Exp Orthop. 2018 Sep 24;5(1):38. doi: 10.1186/s40634-018-0145-5.
8
PRP Treatment Efficacy for Tendinopathy: A Review of Basic Science Studies.富血小板血浆治疗肌腱病的疗效：基础科学研究综述
Biomed Res Int. 2016;2016:9103792. doi: 10.1155/2016/9103792. Epub 2016 Aug 16.
9
Temporal and Spatial Expression of TGF-b1 in the Early Phase of Patellar Tendon Healing after Application of Platelet Rich Plasma.富含血小板血浆应用后髌腱愈合早期TGF-b1的时空表达
Arch Bone Jt Surg. 2016 Apr;4(2):156-60.
10
New and emerging strategies in platelet-rich plasma application in musculoskeletal regenerative procedures: general overview on still open questions and outlook.富血小板血浆在肌肉骨骼再生手术中的新出现策略：关于仍未解决问题及展望的概述
Biomed Res Int. 2015;2015:846045. doi: 10.1155/2015/846045. Epub 2015 May 5.
本文引用的文献
1
The use of autologous PRP gel as an aid in the management of acute trauma wounds.使用自体富血小板凝胶作为急性创伤伤口管理的辅助手段。
Injury. 2009 Aug;40(8):801-5. doi: 10.1016/j.injury.2008.05.002. Epub 2008 Aug 13.
2
Can platelet-rich plasma enhance tendon repair? A cell culture study.富含血小板血浆能否增强肌腱修复？一项细胞培养研究。
Am J Sports Med. 2008 Jun;36(6):1171-8. doi: 10.1177/0363546508314430. Epub 2008 Mar 7.
3
Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing.富含血小板血浆可增强循环来源细胞的初始动员以促进肌腱愈合。
J Cell Physiol. 2008 Jun;215(3):837-45. doi: 10.1002/jcp.21368.
4
Tendon and ligament engineering in the adult organism: mesenchymal stem cells and gene-therapeutic approaches.成年生物体中的肌腱和韧带工程：间充质干细胞与基因治疗方法
Int Orthop. 2007 Dec;31(6):791-7. doi: 10.1007/s00264-007-0395-9. Epub 2007 Jul 19.
5
Stimulation of tendon repair: mechanical loading, GDFs and platelets. A mini-review.肌腱修复的刺激因素：机械负荷、生长分化因子和血小板。一篇综述。
Int Orthop. 2007 Dec;31(6):783-9. doi: 10.1007/s00264-007-0398-6. Epub 2007 Jun 22.
6
Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres.使用富血小板血浆和可生物降解明胶水凝胶微球进行椎间盘再生。
Tissue Eng. 2007 Jan;13(1):147-58. doi: 10.1089/ten.2006.0042.
7
Comparison of surgically repaired Achilles tendon tears using platelet-rich fibrin matrices.使用富血小板纤维蛋白基质对手术修复的跟腱撕裂进行比较。
Am J Sports Med. 2007 Feb;35(2):245-51. doi: 10.1177/0363546506294078. Epub 2006 Nov 12.
8
Platelet-rich plasma (PRP) stimulates the extracellular matrix metabolism of porcine nucleus pulposus and anulus fibrosus cells cultured in alginate beads.富血小板血浆（PRP）刺激在藻酸盐珠中培养的猪髓核和纤维环细胞的细胞外基质代谢。
Spine (Phila Pa 1976). 2006 Apr 20;31(9):959-66. doi: 10.1097/01.brs.0000214942.78119.24.
9
Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in vitro.脱细胞骨髓、富血小板血浆和血清对马悬韧带成纤维细胞的体外合成代谢作用。
Vet Comp Orthop Traumatol. 2006;19(1):43-7.
10
Autologous fibrin matrices: a potential source of biological mediators that modulate tendon cell activities.自体纤维蛋白基质：一种调节肌腱细胞活性的生物介质潜在来源。
J Biomed Mater Res A. 2006 May;77(2):285-93. doi: 10.1002/jbm.a.30585.
Zotero 插件