Orthopaedic Clinic, Democritus University of Thrace, Alexandroupolis, Greece.
Int Orthop. 2010 Feb;34(1):143-8. doi: 10.1007/s00264-009-0728-y. Epub 2009 Feb 11.
A full thickness defect was made in the central portion of the patellar tendon of 48 New Zealand white rabbits. Platelet-rich plasma (PRP) gel was then applied and filled the tendon defect. The same procedure was performed in the control group, without the application of PRP. Animals were sacrificed after one, two, three, and four weeks. Histological and immunohistochemical analyses using a monoclonal antibody against CD31 were performed. The histological examination showed a superior healing process in the PRP group compared with the control group. Especially in the third week, the tissue formed in the PRP group was more mature and dense with less elastic fibres remaining. Neovascularisation was significantly higher in the PRP group during the first two weeks and significantly lower in the third and fourth weeks (p < 0.0001). Histological examination and study of angiogenesis showed that the application of PRP enhances and accelerates the tendon healing process.
在 48 只新西兰白兔的髌腱中央部位制造全层缺损。然后应用富含血小板的血浆（PRP）凝胶并填充腱缺损。在对照组中进行了相同的程序，而没有应用 PRP。动物在 1、2、3 和 4 周后被处死。使用针对 CD31 的单克隆抗体进行组织学和免疫组织化学分析。组织学检查显示 PRP 组的愈合过程优于对照组。特别是在第 3 周，PRP 组形成的组织更成熟且更密集，残留的弹性纤维更少。在最初的两周内，PRP 组的新生血管化明显更高，而在第 3 和第 4 周则明显更低（p<0.0001）。组织学检查和血管生成研究表明，PRP 的应用可增强和加速腱愈合过程。
