Democritus University Hospital of Thrace, Department of Orthopaedic and Trauma Surgery, Alexandroupolis, Dragana, Greece.
Orthop Traumatol Surg Res. 2010 Jun;96(4):381-7. doi: 10.1016/j.otsr.2010.03.010. Epub 2010 May 7.
It is well established that growth factors play a critical role in the healing process of connective tissues. To our knowledge, there are no studies in literature concerning the influence of PRP on growth factors expression.
The aim of this study was to assess the effect of a single application of platelet rich plasma (PRP) gel in a patellar tendon defect on the spatial and temporal expression of Insulin-like Growth Factor 1 (IGF-1) during tendon healing.
Twenty-four animals were randomized to receive PRP (PRPFast, Bioteck) in a gel form (PRP group) and 24 to serve as untreated controls (Control group). A defect of 3 mm x 10 mm was surgically created on the tendon under general anaesthetic and in the PRP group, PRP gel was applied to fill the tendon defect whereas no treatment was applied in the control group. Six animals (12 limbs) from each treatment-group were sacrificed after one, two, three and four weeks following treatment. Histological and immunohistochemical staining were performed.
Histology revealed a faster healing process in the tendons of PRP group in comparison with the controls. In the first 2 weeks of healing, IGF-1 was found intracellularly in various type cells, whereas in the last 2 weeks of healing, IGF-1 was detected mainly in tenocytes. Both cytoplasmic and nuclear expressions were present, whereas the larger amounts of immunoexpression were localized in both epitenon and endotenon. A superior expression of IGF-1 was seen in PRP group compared with controls (p<0.0001) in both the epitenon and endotenon at each time point except at 4th week of healing where a superior expression of IGF-1 was shown in the endotenon of control group, compared to the PRP group (p<0.0001).
PRP may improve tendon defect healing by overexpression of IGF-1.
Laboratory control animal study.
生长因子在结缔组织的愈合过程中起着至关重要的作用，这一点已得到充分证实。据我们所知，目前文献中尚无关于 PRP 对生长因子表达影响的研究。
本研究旨在评估富血小板血浆（PRP）凝胶单次应用于髌腱缺损对腱愈合过程中胰岛素样生长因子 1（IGF-1）时空表达的影响。
24 只动物随机分为接受 PRP（PRPFast，Bioteck）凝胶形式（PRP 组）和未治疗对照组（对照组）。在全身麻醉下，在肌腱上手术造成 3mmx10mm 的缺损，在 PRP 组中，将 PRP 凝胶应用于填充肌腱缺损，而对照组则不进行任何治疗。每组各有 6 只动物（12 条肢体）分别在治疗后 1、2、3 和 4 周处死。进行组织学和免疫组织化学染色。
组织学显示 PRP 组肌腱的愈合过程比对照组更快。在愈合的前 2 周，IGF-1 在内皮细胞和各种类型的细胞中被发现，而在愈合的最后 2 周，IGF-1 主要在腱细胞中被检测到。细胞质和核表达均存在，而大量的免疫表达位于腱上皮和腱内膜中。与对照组相比，PRP 组在每个时间点的腱上皮和腱内膜中 IGF-1 的表达均较高（p<0.0001），除了在愈合的第 4 周，对照组的腱内膜中 IGF-1 的表达高于 PRP 组（p<0.0001）。
PRP 通过过度表达 IGF-1 可能改善腱缺损的愈合。
实验室对照动物研究。
