Segev Osnat, Meusel Wolfram, Friedenberger Melanie, Brenner Asher, Kushmaro Ariel
Unit of Environmental Engineering, Faculty of Engineering Sciences, Ben-Gurion University of the Negev, P.O. Box 653, 84105, Be'er-Sheva, Israel.
Biodegradation. 2009 Sep;20(5):621-7. doi: 10.1007/s10532-009-9249-z. Epub 2009 Feb 10.
Halogenated organic compounds constitute one of the largest and most diverse groups of chemicals in the environment. Many of these compounds are toxic, persistent and, as a result of their often limited biodegradability, tend to bioaccumulate in the environment. Dibromoneopentyl glycol (DBNPG) and tribromoneopentyl alcohol (TBNPA) are brominated flame retardants commonly used as additives during the manufacture of plastic polymers and as chemical intermediates in the synthesis of other flame retardants. Both are classified as not readily biodegradable. In this paper, we demonstrate the biodegradation of both DBNPG and TBNPA by a common bacterial consortium under aerobic conditions in enrichment cultures containing yeast extract. DBNPG and TBNPA biodegradation is accompanied by a release of bromide into the medium, due to a biological debromination reaction. Molecular analysis of the clone library PCR amplified 16S rRNA gene was used to characterize the bacterial consortium involved in the biodegradation.
卤代有机化合物是环境中数量最多、种类最丰富的化学物质类别之一。这些化合物中的许多都具有毒性、持久性，并且由于其通常有限的生物降解性，往往会在环境中生物累积。二溴新戊二醇（DBNPG）和三溴新戊醇（TBNPA）是溴化阻燃剂，通常在塑料聚合物制造过程中用作添加剂，并在其他阻燃剂的合成中用作化学中间体。两者都被归类为难生物降解物质。在本文中，我们证明了在含有酵母提取物的富集培养物中，一种常见的细菌群落可在有氧条件下对DBNPG和TBNPA进行生物降解。由于生物脱溴反应，DBNPG和TBNPA的生物降解伴随着溴化物释放到培养基中。利用对克隆文库PCR扩增的16S rRNA基因进行分子分析，来表征参与生物降解的细菌群落。
