Reed Jason, Troke Joshua J, Schmit Joanna, Han Sen, Teitell Michael A, Gimzewski James K
Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles Young Drive East, Los Angeles, California 90095, USA.
ACS Nano. 2008 May;2(5):841-6. doi: 10.1021/nn700303f.
Cancer and many other diseases are characterized by changes in cell morphology, motion, and mechanical rigidity. However, in live cell cytology, stimulus-induced morphologic changes typically take 10-30 min to detect. Here, we employ live-cell interferometry (LCI) to visualize the rapid response of a whole cell to mechanical stimulation, on a time scale of seconds, and we detect cytoskeletal remodeling behavior within 200 s. This behavior involved small, rapid changes in cell content and miniscule changes in shape; it would be difficult to detect with conventional or phase contrast microscopy alone and is beyond the dynamic capability of AFM. We demonstrate that LCI provides a rapid, quantitative reconstruction of the cell body with no labeling. This is an advantage over traditional microscopy and flow cytometry, which require cell surface tagging and/or destructive cell fixation for labeling.
癌症和许多其他疾病的特征在于细胞形态、运动和机械刚性的变化。然而，在活细胞细胞学中，刺激诱导的形态变化通常需要10 - 30分钟才能检测到。在这里，我们采用活细胞干涉测量法（LCI）在秒级时间尺度上可视化整个细胞对机械刺激的快速反应，并且我们在200秒内检测到细胞骨架重塑行为。这种行为涉及细胞内容物的微小、快速变化以及形状的微小变化；仅用传统显微镜或相差显微镜很难检测到，并且超出了原子力显微镜（AFM）的动态能力。我们证明LCI无需标记即可对细胞体进行快速、定量的重建。这相对于传统显微镜和流式细胞术具有优势，传统方法需要对细胞表面进行标记和/或进行破坏性的细胞固定来进行标记。
