活细胞质量谱分析：定量生物物理学中的一种新兴方法。
Live-cell mass profiling: an emerging approach in quantitative biophysics.
机构信息
Department of Bioengineering, University of California, Los Angeles (UCLA), Los Angeles, California, USA.
1] Department of Bioengineering, University of California, Los Angeles (UCLA), Los Angeles, California, USA. [2] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA. [3] California NanoSystems Institute, UCLA, Los Angeles, California, USA. [4] Broad Stem Cell Research Center, UCLA, Los Angeles, California, USA. [5] Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, California, USA. [6] Molecular Biology Institute, UCLA, Los Angeles, California, USA.
出版信息
Nat Methods. 2014 Dec;11(12):1221-8. doi: 10.1038/nmeth.3175.
Cell mass, volume and growth rate are tightly controlled biophysical parameters in cellular development and homeostasis, and pathological cell growth defines cancer in metazoans. The first measurements of cell mass were made in the 1950s, but only recently have advances in computer science and microfabrication spurred the rapid development of precision mass-quantifying approaches. Here we discuss available techniques for quantifying the mass of single live cells with an emphasis on relative features, capabilities and drawbacks for different applications.
细胞质量、体积和生长速率是细胞发育和内稳态中受到严格控制的生物物理参数，病理性细胞生长定义了后生动物中的癌症。细胞质量的首次测量是在 20 世纪 50 年代进行的，但直到最近，计算机科学和微制造的进步才推动了精确质量定量方法的快速发展。在这里，我们讨论了用于定量测量单个活细胞质量的现有技术，重点介绍了不同应用的相对特征、功能和缺点。
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