Pornprasert Sakorn, Praparattanapan Jutarat, Khamwan Chantana, Pawichai Sudjai, Pimsarn Parichat, Samleerat Tanawan, Leechanachai Pranee, Supparatpinyo Khunchai
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand.
Mycoses. 2009 Nov;52(6):487-92. doi: 10.1111/j.1439-0507.2008.01653.x. Epub 2009 Jan 9.
Penicillium marneffei is a dimorphic fungus, which is endemic in Southeast Asia and responsible for emerging opportunistic infections. Diagnosis of penicilliosis may be difficult when few yeast cells are present, while a gold standard diagnosis technique requires long-term culture. In order to provide a more rapid and accurate diagnosis, we developed a TaqMan real-time PCR to detect and identify P. marneffei DNA coding for 5.8S rRNA in purified yeast DNA and clinical samples. All P. marneffei DNA preparations could be detected using specific primers and TaqMan probe. The assay has a sensitivity to detect at least 10 yeast cells in seeded blood. Moreover, it can detect P. marneffei DNA in peripheral blood samples and blood-culture bottles. Therefore, the real-time PCR assay may represent a potential tool for early diagnosis of penicilliosis marneffei.
马尔尼菲青霉菌是一种双相真菌，在东南亚地区为地方性真菌，可引发机会性感染。当酵母细胞数量较少时，马尔尼菲青霉病的诊断可能会很困难，而金标准诊断技术需要长期培养。为了提供更快速准确的诊断方法，我们开发了一种TaqMan实时荧光定量PCR技术，用于在纯化的酵母DNA和临床样本中检测和鉴定编码5.8S核糖体RNA的马尔尼菲青霉菌DNA。使用特异性引物和TaqMan探针可检测到所有马尔尼菲青霉菌DNA样本。该检测方法能够检测出接种血液中至少10个酵母细胞，还能检测外周血样本和血培养瓶中的马尔尼菲青霉菌DNA。因此，实时荧光定量PCR检测方法可能是早期诊断马尔尼菲青霉病的一种潜在工具。
