富血小板纤维蛋白、浓缩生长因子和血小板减少血浆对兔拔牙窝愈合影响的比较研究。

A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits.

机构信息

Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, 646000, China.

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, 646000, China.

出版信息

BMC Oral Health. 2022 Mar 23;22(1):87. doi: 10.1186/s12903-022-02126-0.

BACKGROUND

Autologous platelet concentrate has been widely used to encourage the regeneration of hard and soft tissues. Up to now, there are three generations of autologous platelet concentrates. Many studies have shown that the three autologous concentrates have different effects, but the specific diversities have not been studied. The purpose of this study was to explore and compare the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in New Zealand rabbits.

METHODS

A total of 24 healthy male New Zealand white rabbits aged 8-12 weeks were selected. The experimental animals were randomly divided into four groups: three experimental groups were respectively implanted with PPP, CGF and PRF gel after bilateral mandibular anterior teeth were extracted, and the control group did not implant any material. The alveolar bone of the mandibular anterior region was taken at 2, 4 and 8 weeks after operation. The height and width of the extraction wound were detected by CBCT, the growth of the new bone was observed by HE and Masson staining, and the expression of osteogenic genes was detected by real-time PCR. Data were analyzed using IBM SPSS statistical package 22.0.

RESULTS

The radiological results showed that alveolar bone resorption in all groups gradually increased over time. However, the experimental groups showed lower amounts of bone resorption. The histological results showed that new bone formation was observed in all groups. Over time, the new bone trabeculae of the CGF group became closely aligned while those in the PPP and PRF groups remained scattered. PCR results showed that the expression of BMP-2 and ALP was higher in the experimental groups than the control group.

CONCLUSION

In conclusion, the application of PRF, CGF and PPP in tooth extraction sockets effectively promoted bone regeneration. CGF showed more effective bone induction and tissue regeneration ability in the long term.

背景

自体血小板浓缩物已被广泛用于促进硬组织和软组织的再生。到目前为止，已经有三代自体血小板浓缩物。许多研究表明，这三种自体浓缩物具有不同的效果，但具体的差异尚未得到研究。本研究旨在探讨和比较富血小板纤维蛋白、浓缩生长因子和血小板贫血浆对新西兰兔拔牙窝愈合的影响。

方法

共选取 24 只 8-12 周龄健康雄性新西兰白兔，将实验动物随机分为四组：三组实验组分别在双侧下颌前牙拔除后植入 PPP、CGF 和 PRF 凝胶，对照组不植入任何材料。术后 2、4、8 周取下颌前区牙槽骨，CBCT 检测拔牙创的高度和宽度，HE 和 Masson 染色观察新骨生长情况，实时 PCR 检测成骨基因的表达。采用 IBM SPSS 统计软件包 22.0 进行数据分析。