Reichert Johannes C, Saifzadeh Siamak, Wullschleger Martin E, Epari Devakara R, Schütz Michael A, Duda Georg N, Schell Hanna, van Griensven Martijn, Redl Heinz, Hutmacher Dietmar W
Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 60 Musk Avenue, Kelvin Grove, Qld 4059, Australia.
Biomaterials. 2009 Apr;30(12):2149-63. doi: 10.1016/j.biomaterials.2008.12.050. Epub 2009 Feb 10.
A considerable number of international research groups as well as commercial entities work on the development of new bone grafting materials, carriers, growth factors and specifically tissue-engineered constructs for bone regeneration. They are strongly interested in evaluating their concepts in highly reproducible large segmental defects in preclinical and large animal models. To allow comparison between different studies and their outcomes, it is essential that animal models, fixation devices, surgical procedures and methods of taking measurements are well standardized to produce reliable data pools and act as a base for further directions to orthopaedic and tissue engineering developments, specifically translation into the clinic. In this leading opinion paper, we aim to review and critically discuss the different large animal bone defect models reported in the literature. We conclude that most publications provide only rudimentary information on how to establish relevant preclinical segmental bone defects in large animals. Hence, we express our opinion on methodologies to establish preclinical critically sized, segmental bone defect models used in past research with reference to surgical techniques, fixation methods and postoperative management focusing on tibial fracture and segmental defect models.
相当数量的国际研究团队以及商业实体致力于开发新型骨移植材料、载体、生长因子，特别是用于骨再生的组织工程构建体。他们对在临床前和大型动物模型的高度可重复的大段骨缺损中评估其概念非常感兴趣。为了能够比较不同研究及其结果，动物模型、固定装置、手术程序和测量方法必须得到很好的标准化，以产生可靠的数据集，并作为骨科和组织工程发展进一步方向的基础，特别是转化到临床应用。在这篇主导性观点论文中，我们旨在回顾并批判性地讨论文献中报道的不同大型动物骨缺损模型。我们得出结论，大多数出版物仅提供了关于如何在大型动物中建立相关临床前节段性骨缺损的基本信息。因此，我们参考手术技术、固定方法和术后管理，特别是针对胫骨骨折和节段性缺损模型，对过去研究中用于建立临床前临界尺寸节段性骨缺损模型的方法发表我们的意见。
