含蜂胶漱口水对口腔微生物及人牙龈成纤维细胞的影响。
Effect of mouthrinse containing propolis on oral microorganisms and human gingival fibroblasts.
作者信息
Ozan Fatih, Sümer Zeynep, Polat Zübeyde Akın, Er Kürşat, Ozan Ulkü, Deger Orhan
机构信息
Department of Oral and Maxillofacial Surgery, School of Dentistry, Cumhuriyet University, Sivas, Turkey.
出版信息
Eur J Dent. 2007 Oct;1(4):195-201.
Abstract
OBJECTIVES
The aim of this study was to compare the effects of four different mouthrinse containing propolis solutions and mouthrinse containing 0.2% chlorhexidine (CHX) on oral microorganisms and human gingival fibroblasts.
METHODS
Four different solutions of propolis were prepared and propylene glycol and alcohol were used as solvents for each propolis sample. Mouthrinse containing propolis was prepared at four different concentrations as 10%, 5%, 2.5% and 1%. Besides, CHX was used as control group. The antibacterial effects of five solutions on oral microorganisms were tested and their cytotoxic effects on human gingival fibroblasts were evaluated by agar diffusion test.
RESULTS
At this concentrations effectiveness of mouthrinse containing propolis samples on oral microorganisms were not found as effective as CHX. On the contrary, samples found less cytotoxic on human gingival fibroblasts than CHX.
CONCLUSIONS
Standardized preparations of propolis can be used as a mouthrinse at appropriate concentrations. To obtain a standardized chemical composition, advanced researches are needed.
摘要
目的
本研究旨在比较四种不同的含蜂胶漱口水溶液和含0.2%氯己定（CHX）漱口水对口腔微生物和人牙龈成纤维细胞的影响。
方法
制备四种不同的蜂胶溶液，每种蜂胶样品分别使用丙二醇和乙醇作为溶剂。制备含蜂胶漱口水的四种不同浓度分别为10%、5%、2.5%和1%。此外，将CHX用作对照组。通过琼脂扩散试验测试了五种溶液对口腔微生物的抗菌作用，并评估了它们对人牙龈成纤维细胞的细胞毒性作用。
结果
在此浓度下，含蜂胶样品的漱口水对口腔微生物的有效性不如CHX。相反，样品对人牙龈成纤维细胞的细胞毒性比CHX小。
结论
标准化的蜂胶制剂可在适当浓度下用作漱口水。为了获得标准化的化学成分，需要进行进一步的研究。
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本文引用的文献
1
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J Ethnopharmacol. 2005 Dec 1;102(3):371-6. doi: 10.1016/j.jep.2005.06.035. Epub 2005 Aug 3.
2
In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development.巴西蜂胶分离组分对龋齿发展的体内外效应
J Ethnopharmacol. 2005 Oct 3;101(1-3):110-5. doi: 10.1016/j.jep.2005.04.001.
3
Effect of various chlorhexidine regimens on salivary bacteria and de novo plaque formation.不同洗必泰方案对唾液细菌和新发牙菌斑形成的影响。
J Clin Periodontol. 2003 Oct;30(10):919-25. doi: 10.1034/j.1600-051x.2003.00420.x.
4
The effects of a new mouthrinse containing chlorhexidine, cetylpyridinium chloride and zinc lactate on the microflora of oral halitosis patients: a dual-centre, double-blind placebo-controlled study.含氯己定、西吡氯铵和乳酸锌的新型漱口水对口腔口臭患者微生物群的影响：一项双中心、双盲、安慰剂对照研究。
J Clin Periodontol. 2003 May;30(5):427-34. doi: 10.1034/j.1600-051x.2003.20004.x.
5
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6
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Am J Chin Med. 2002;30(2-3):245-54. doi: 10.1142/S0192415X02000387.
7
Microbiology and treatment of halitosis.口臭的微生物学与治疗
Periodontol 2000. 2002;28:256-79. doi: 10.1034/j.1600-0757.2002.280111.x.
8
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Periodontol 2000. 2002;28:12-55. doi: 10.1034/j.1600-0757.2002.280102.x.
9
Seasonal effect on Brazilian propolis antibacterial activity.
J Ethnopharmacol. 2000 Nov;73(1-2):243-9. doi: 10.1016/s0378-8741(00)00320-2.
10
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Arch Oral Biol. 2000 Feb;45(2):141-8. doi: 10.1016/s0003-9969(99)00117-x.
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