Sonmez Sule, Kirilmaz Levent, Yucesoy Mine, Yücel Banu, Yilmaz Berna
Ege University, School of Dentistry, Department of Periodontology, Bornova, Izmir, Turkey.
J Ethnopharmacol. 2005 Dec 1;102(3):371-6. doi: 10.1016/j.jep.2005.06.035. Epub 2005 Aug 3.
Propolis is one of the few natural remedies that have maintained its popularity over a long period of time. The aim of this study is to investigate the antimicrobial properties of six propolis solutions and evaluate their cytotoxicity on gingival fibroblasts at different dilutions. Two different solutions of powder propolis (Sigma) and Turkish propolis were prepared and propylene glycol (PG) and alcohol were used as solvents for each propolis sample. In addition to the four propolis solutions, two other propolis samples of far geographic regions (USA and Australia) were included in the study. The antibacterial effects of six solutions on oral pathogen microorganisms were tested and their cytotoxic effects on human gingival fibroblasts were evaluated by MTT assay. The effective dilutions of the six propolis samples on periodontopathogen microorganisms were found to be cytotoxic to gingival fibroblasts. All solutions had strong antifungal activity and the effective dilutions were safe for gingival fibroblasts. Propolis could have a promising role in the future medicine, if appropriate solutions can be prepared being strongly antibacterial and non-cytotoxic as well.
蜂胶是少数长期以来一直广受欢迎的天然药物之一。本研究的目的是调查六种蜂胶溶液的抗菌特性，并评估它们在不同稀释度下对牙龈成纤维细胞的细胞毒性。制备了两种不同的粉状蜂胶（Sigma）溶液和土耳其蜂胶溶液，每种蜂胶样品均使用丙二醇（PG）和酒精作为溶剂。除了这四种蜂胶溶液外，研究还纳入了来自遥远地理区域（美国和澳大利亚）的另外两种蜂胶样品。测试了六种溶液对口腔致病微生物的抗菌作用，并通过MTT法评估了它们对人牙龈成纤维细胞的细胞毒性作用。发现六种蜂胶样品对牙周致病微生物的有效稀释度对牙龈成纤维细胞具有细胞毒性。所有溶液均具有很强的抗真菌活性，且有效稀释度对牙龈成纤维细胞是安全的。如果能够制备出具有强抗菌性且无细胞毒性的合适溶液，蜂胶在未来医学中可能会发挥重要作用。
