[干扰素的超诱导及其信使核糖核酸的研究]
[Superinduction of interferon and and a study of its messenger RNA].
作者信息
Ershov F I, Sokolova T M, Kadyrova A A
出版信息
Antibiotiki. 1977 Mar;22(3):247-52.
Combined use of interferon inductor poly-IC and antibiotics (cycloheximide and actinomycin D) provided a significant increase (up to 1000 times) in interferon production by chick, mouse, monkey and human cells. Messenger RNA with matrix activity for interferon (mRNA-IF) was isolated from superinduced cells. On translation of mRNA-IF in homogenous and heterogenous cells the specificity of interferons produced was determined by the type of the cells from which mRNA-IF was isolated. Sedimentation analysis of various mRNA-IF revealed 2 peaks of activity: major (5--15S) and minor (25--30S).
干扰素诱导剂聚肌胞苷酸（poly-IC）与抗生素（放线菌酮和放线菌素D）联合使用，可使鸡、小鼠、猴和人类细胞产生的干扰素显著增加（高达1000倍）。从超诱导细胞中分离出具有干扰素模板活性的信使核糖核酸（mRNA-IF）。在同源和异源细胞中对mRNA-IF进行翻译时，所产生干扰素的特异性取决于分离mRNA-IF的细胞类型。对各种mRNA-IF的沉降分析显示有两个活性峰：主要峰（5-15S）和次要峰（25-30S）。
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