Stagg C H, Wallis C, Ward C H
Appl Environ Microbiol. 1977 Feb;33(2):385-91. doi: 10.1128/aem.33.2.385-391.1977.
The model system consisted of bacteriophage MS-2, bentonite clay, and hypochlorous acid (HOC1). Factors that influenced association of the bacterial virus with bentonite were the titer of unadsorbed viruses, clay concentration, cation concentration, temperature, stirring rate, and the presence of soluble organics. Variation of the kinetic adsorption rate constant with stirring speed indicates that phage attachment is a diffusion-limited process; the attachment reaction has an apparent activation energy of 1 kcal/mol. About 18% of clay-associated bacteriophages was recovered by mixing the suspension with an organic eluent. Inactivation data were obtained from batch reactors operated under those conditions in which loss of HOC1 was minimal during the reaction. Bacteriophages attached to clay were more resistant to HOC1 than were freely suspended phages; for equivalent HOC1 concentrations, clay-associated phages required about twice the time that freely suspended phages required for loss of 99% of the initial virus titer.
该模型系统由噬菌体MS-2、膨润土和次氯酸（HOC1）组成。影响细菌病毒与膨润土结合的因素有未吸附病毒的滴度、粘土浓度、阳离子浓度、温度、搅拌速率以及可溶性有机物的存在。动力学吸附速率常数随搅拌速度的变化表明噬菌体附着是一个扩散受限过程；附着反应的表观活化能为1千卡/摩尔。通过将悬浮液与有机洗脱液混合，回收了约18%与粘土结合的噬菌体。失活数据是在反应过程中HOC1损失最小的条件下，从间歇式反应器中获得的。附着在粘土上的噬菌体比自由悬浮的噬菌体对HOC1更具抗性；对于相同的HOC1浓度，与粘土结合的噬菌体失去99%初始病毒滴度所需的时间约为自由悬浮噬菌体所需时间的两倍。
