废水中与固体物质相关的病毒及污泥中的病毒检测
Demonstration of solids-associated virus in wastewater and sludge.
作者信息
Wellings F M, Lewis A L, Mountain C W
出版信息
Appl Environ Microbiol. 1976 Mar;31(3):354-8. doi: 10.1128/aem.31.3.354-358.1976.
Abstract
Data presented demonstrate the relatively high multiplicity of solids-associated virus in field samples, i.e., wastewater, sludge, and soils. Influent, effluent, and chlorinated effluent samples showed 16.1 to 100% of the total virus demonstrated in samples to be solids associated. Three techniques for freeing solids-associated virus are described and compared. Using sonication of solids and polyethylene glycol concentration, virus was demonstrated in fully digested sludge (60 days at 34 C), sand at the site of a sewer leak, and dried sludge cake and mud 900 m downstream from a sewage disposal site. These data emphasize the inadequacy of virus concentration techniques that do not include the processing of solids. In situ elution failed to free solids-associated virus.
摘要
所呈现的数据表明，在现场样本（即废水、污泥和土壤）中，与固体相关的病毒具有相对较高的多样性。进水、出水和氯化后出水样本显示，样本中总病毒的16.1%至100%与固体相关。文中描述并比较了三种释放与固体相关病毒的技术。通过对固体进行超声处理和聚乙二醇浓缩，在完全消化的污泥（34℃下60天）、下水道泄漏处的沙子、以及污水处理场下游900米处的干污泥饼和泥浆中检测到了病毒。这些数据强调了不包括固体处理的病毒浓缩技术的不足。原位洗脱未能释放与固体相关的病毒。
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