使用Ni2+三（氮川三乙酸）量子点共轭物对活细胞中单个组氨酸标签蛋白进行高亲和力标记和追踪。
High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ tris-nitrilotriacetic acid quantum dot conjugates.
作者信息
Roullier Victor, Clarke Samuel, You Changjiang, Pinaud Fabien, Gouzer G Géraldine, Schaible Dirk, Marchi-Artzner Valérie, Piehler Jacob, Dahan Maxime
机构信息
Université de Rennes, Sciences Chimiques de Rennes, CNRS UMR 6226, Campus de Beaulieu, 35042 Rennes Cedex, France.
出版信息
Nano Lett. 2009 Mar;9(3):1228-34. doi: 10.1021/nl9001298.
Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for QD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates tris-nitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using QD-trisNTA, we demonstrate two-color QD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein-protein interactions at the single molecule level.
使用荧光量子点（QD）对许多细胞过程进行研究受到QD表面功能化和靶向的复杂要求的阻碍。为应对这些挑战，我们设计、表征并应用了QD-三氮杂环壬四乙酸（QD-trisNTA），它整合了三氮杂环壬四乙酸，这是一种用于普遍存在的多组氨酸蛋白标签的小型高亲和力识别单元。使用QD-trisNTA，我们在活细胞中展示了1型干扰素受体亚基的双色QD追踪，这有可能在单分子水平上直接可视化蛋白质-蛋白质相互作用。
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