Tan Lijun, Ren Yijin, Wang Jun, Jiang Lingyong, Cheng Hui, Sandham Andrew, Zhao Zhihe
Sichuan University, Sichuan, China.
Angle Orthod. 2009 Mar;79(2):292-8. doi: 10.2319/031608-150.1.
To test the null hypothesis that increased tooth displacement in ovariectomized rats is not related to differential expressions of OPG and RANKL in the periodontium.
Eighty-four 12-week female rats were used; half were ovariectomized and half were not. Three months later, the maxillary first molar was moved mesially. Groups of rats were sacrificed at days 0, 1, 3, 5, 7, 10, and 14 after activation. Tooth movement was measured at each time point. OPG and RANKL expressions were examined through immunohistochemistry.
Ovariectomized and nonovariectomized rats showed three-phase tooth movement. In both groups, OPG expression increased at the tension area and RANKL increased at the pressure area. The OPG/RANKL ratio coincided with tooth movement, especially in the linear phase from 7 to 14 days.
The null hypothesis is rejected. The increased rate of tooth movement in ovariectomized rats was related to differential expressions of OPG and RANKL.
检验卵巢切除大鼠牙齿移位增加与牙周组织中骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）的差异表达无关这一零假设。
选用84只12周龄雌性大鼠，其中一半进行卵巢切除，另一半未切除。三个月后，将上颌第一磨牙向近中移动。在激活后的第0、1、3、5、7、10和14天处死大鼠组。在每个时间点测量牙齿移动情况。通过免疫组织化学检测OPG和RANKL的表达。
卵巢切除和未切除卵巢的大鼠均表现出三相牙齿移动。在两组中，OPG表达在张力区增加，RANKL在压力区增加。OPG/RANKL比值与牙齿移动一致，尤其是在第7至14天的线性期。
零假设被拒绝。卵巢切除大鼠牙齿移动速率增加与OPG和RANKL的差异表达有关。
