Tigano Concetta, Tomasello Barbara, Pulvirenti Valentina, Ferrito Venera, Copat Chiara, Carpinteri Guido, Mollica Emanuele, Sciacca Salvatore, Renis Marcella
Department of Animal Biology M. La Greca, University of Catania, Via Androne 81, 95124 Catania, Italy.
Ecotoxicol Environ Saf. 2009 May;72(4):1278-86. doi: 10.1016/j.ecoenv.2008.09.028. Epub 2009 Feb 13.
The blenny Parablennius sanguinolentus was selected as a useful bioindicator of environmental pollution. Chemical parameters in water and sediments from three different sampling sites along the Sicilian Ionian coast were determined and metal concentrations in fish muscle were measured. DNA fragmentation and oxidation in erythrocytes and hepatocytes was determined by the Comet assay and HSP70 expression levels were evaluated in the liver. The results show an increased level of chromium in sediments and high polycyclic aromatic hydrocarbon (PAH) concentrations in water at one site. The bioaccumulation of metals in muscle tissue shows high concentrations of lead in some samples. A high percentage of DNA damage in blood and liver cells, as well as high hepatic levels of HSP70, were found in all the sites. The results demonstrate the usefulness of an integrated chemical and biological approach for the determination of environmental stress.
黏鱼拟斑背隆头鱼被选为环境污染的有效生物指标。测定了西西里岛爱奥尼亚海岸三个不同采样点水和沉积物中的化学参数，并测量了鱼肌肉中的金属浓度。通过彗星试验测定红细胞和肝细胞中的DNA片段化和氧化情况，并评估肝脏中HSP70的表达水平。结果显示，一个采样点的沉积物中铬含量增加，水中多环芳烃（PAH）浓度较高。肌肉组织中金属的生物积累显示，一些样本中铅含量很高。在所有采样点均发现血液和肝细胞中DNA损伤比例较高，以及肝脏中HSP70水平较高。结果表明，综合化学和生物学方法在确定环境压力方面是有用的。
