Zimmermann Benno F, Papagiannopoulos Menelaos, Brachmann Sonja, Lorenz Mario, Stangl Verena, Galensa Rudolf
University of Bonn, Institute of Nutrition and Food Sciences, Endenicher Allee 11-13, 53115 Bonn, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Mar 15;877(8-9):823-6. doi: 10.1016/j.jchromb.2009.01.013. Epub 2009 Jan 21.
This paper describes a new and straightforward method for determination of the green tea catechins epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin gallate in human plasma. Sample preparation includes addition only of dimethylformamide and trichloroacetic acid. After centrifugation, the supernatant can be injected into the HPLC. If required, the glucuronides and sulphates of the catechins can be enzymatically hydrolysed before extraction. Recovery ranges from 92.9 to 98.2%; limits of detection, from 2.4 to 5.0 ng/mL; and relative standard deviations, from 3.1 to 8.6%. Twelve samples can be processed within 45 min, and are then ready to be injected into the HPLC. The method was successfully applied to human plasma. This method is suitable for studies on absorption, bioavailability, and kinetics of green tea catechins in plasma. Since manual work and time consumption are minimal, the procedure is especially useful for large numbers of samples.
本文描述了一种测定人血浆中绿茶儿茶素表儿茶素、表儿茶素没食子酸酯、表没食子儿茶素和表没食子儿茶素没食子酸酯的新的简便方法。样品制备仅包括添加二甲基甲酰胺和三氯乙酸。离心后，上清液可注入高效液相色谱仪。如有需要，儿茶素的葡萄糖醛酸苷和硫酸盐可在萃取前进行酶促水解。回收率在92.9%至98.2%之间；检测限在2.4至5.0 ng/mL之间；相对标准偏差在3.1%至8.6%之间。45分钟内可处理12个样品，然后即可注入高效液相色谱仪。该方法已成功应用于人血浆。该方法适用于研究血浆中绿茶儿茶素的吸收、生物利用度和动力学。由于手工操作和时间消耗极少，该程序对大量样品特别有用。
