Meyer J, Walker-Jonah A, Hollenberg C P
Institut für Mikrobiologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
Mol Cell Biol. 1991 Nov;11(11):5454-61. doi: 10.1128/mcb.11.11.5454-5461.1991.
We have analyzed a GAL1 mutant (gal1-r strain) of the yeast Kluyveromyces lactis which lacks the induction of beta-galactosidase and the enzymes of the Leloir pathway in the presence of galactose. The data show that the K. lactis GAL1 gene product has, in addition to galactokinase activity, a function required for induction of the lactose system. This regulatory function is not dependent on galactokinase activity, as it is still present in a galactokinase-negative mutant (gal1-209). Complementation studies in Saccharomyces cervisiae show that K. lactis GAL1 and gal1-209, but not gal1-r, complement the gal3 mutation. We conclude that the regulatory function of GAL1 in K. lactis soon after induction is similar to the function of GAL3 in S. cerevisiae.
我们分析了乳酸克鲁维酵母的一个GAL1突变体（gal1-r菌株），该突变体在半乳糖存在的情况下缺乏β-半乳糖苷酶的诱导以及Leloir途径的酶。数据表明，乳酸克鲁维酵母GAL1基因产物除了具有半乳糖激酶活性外，还具有诱导乳糖系统所需的功能。这种调节功能不依赖于半乳糖激酶活性，因为它在半乳糖激酶阴性突变体（gal1-209）中仍然存在。在酿酒酵母中的互补研究表明，乳酸克鲁维酵母GAL1和gal1-209，但不是gal1-r，可互补gal3突变。我们得出结论，诱导后不久GAL1在乳酸克鲁维酵母中的调节功能类似于GAL3在酿酒酵母中的功能。
