Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, A.P. 70228, México D.F 04510, México.
Int J Biol Sci. 2011;7(9):1287-97. doi: 10.7150/ijbs.7.1287. Epub 2011 Oct 25.
Cysticercosis caused by Taenia solium is a health threat for humans and pigs living in developing countries, for which there is neither a flawless immunodiagnostic test nor a totally effective vaccine. Suspecting of individual diversity of hosts and parasites as possible sources of the variations of the parasite loads among cysticercotic animals and of the limited success of such immunological applications as well as, we explored and measured both in nine cases of naturally acquired porcine cysticercosis. For this purpose, 2-Dimensional IgG immunoblots were performed by reacting the sera of each cysticercotic pig with the antigens contained in the vesicular fluid (VF) of their own cysticerci. We found an unexpectedly large diversity among the proteins and antigens contained in each of the nine VFs. Also diverse were the serum IgG antibody responses of the nine pigs, as none of their 2D- immunoblot images exhibited the same number of spots and resembled each other in only 6.3% to 65.3% of their features. So large an individual immunological diversity of the cysticercal antigens and of the infected pigs´ IgG antibody response should be taken into account in the design of immunological tools for diagnosis and prevention of cysticercosis and should also be considered as a possibly significant source of diversity in Taenia solium´s infectiveness and pathogenicity.
猪带绦虫引起的囊尾蚴病对生活在发展中国家的人类和猪构成健康威胁，目前既没有完美的免疫诊断测试，也没有完全有效的疫苗。由于怀疑宿主和寄生虫个体多样性可能是囊尾蚴动物中寄生虫负荷变异的原因，以及这些免疫学应用的有限成功，我们在 9 例自然获得性猪囊尾蚴病中进行了探索和测量。为此，通过将每个囊尾蚴猪的血清与自身囊尾蚴的囊液 (VF) 中的抗原进行反应，进行了 2-D IgG 免疫印迹。我们发现，每 9 个 VF 中所含的蛋白质和抗原都存在出乎意料的多样性。9 头猪的血清 IgG 抗体反应也存在多样性，因为它们的 2D 免疫印迹图像没有一个显示相同数量的斑点，并且只有 6.3%到 65.3%的特征彼此相似。因此，囊尾蚴抗原和受感染猪的 IgG 抗体反应的个体免疫多样性如此之大，在设计用于诊断和预防囊尾蚴病的免疫学工具时应予以考虑，并且还应被视为绦虫感染性和致病性多样性的一个可能重要来源。
