Institut Pasteur de Madagascar, BP 1274 Antananarivo 101, Madagascar.
Diagn Microbiol Infect Dis. 2012 Jan;72(1):85-9. doi: 10.1016/j.diagmicrobio.2011.09.014. Epub 2011 Nov 15.
This study aimed to evaluate 5 enzyme immunoassays for detecting human antibodies against Taenia solium in human serum and for the diagnosis of neurocysticercosis (NCC): DRG™, RIDASCREEN™, NOVATECH™, CYPRESS™, and IVD™. A collection of 114 reference serum samples were used. All sera were tested both by ELISA and by an immunoblot method (enzyme-linked immunoelectrotransfer blot [EITB]). When compared with EITB, the Ridascreen™ test had the best positive concordance rate (85.1-91.2%) and the NovaLisa test™ showed the optimal negative concordance rate (93.7-95.6%). All tests had a sensitivity under 72% and a specificity above 60%. The best sensitivity was obtained using Ridascreen™ test (71.4%). An optimal specificity was achieved by the NovaLisa test™. T. solium-positive sera all cross-reacted with E. granulosus positive samples. In the commercial assays evaluated here, the most appropriate ELISA test for screening may be the Ridascreen™ assay. Antibody detection seems to be not appropriate for NCC diagnosis because of its overall lack of sensitivity.
本研究旨在评估 5 种酶联免疫吸附试验（ELISA）检测人血清中抗猪带绦虫抗体，用于诊断神经囊尾蚴病（NCC）：DRG™、RIDASCREEN™、NOVATECH™、CYPRESS™ 和 IVD™。使用了 114 份参考血清样本。所有血清均通过 ELISA 和免疫印迹法（酶联免疫电转移印迹 [EITB]）进行检测。与 EITB 相比，Ridascreen™ 检测的阳性符合率最高（85.1-91.2%），而 NovaLisa 检测™的阴性符合率最佳（93.7-95.6%）。所有检测的敏感性均低于 72%，特异性均高于 60%。Ridascreen™ 检测的敏感性最佳（71.4%）。NovaLisa 检测™ 的特异性最佳。T. solium 阳性血清均与 E. granulosus 阳性样本发生交叉反应。在评估的商业检测中，最适合筛查的 ELISA 检测可能是 Ridascreen™ 检测。由于敏感性不足，抗体检测似乎不适合 NCC 诊断。
