Breitling Frank, Nesterov Alexander, Stadler Volker, Felgenhauer Thomas, Bischoff F Ralf
German Cancer Research Center, Im Neuenheimer Feld 580, Heidelberg, Germany.
Mol Biosyst. 2009 Mar;5(3):224-34. doi: 10.1039/b819850k. Epub 2009 Jan 16.
Arrays promise to advance biology by allowing parallel screening for many different binding partners. Meanwhile, lithographic methods enable combinatorial synthesis of > 50,000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This review discusses recent developments in the field with an emphasis on methods that lead to very high-density peptide arrays.
阵列有望通过对许多不同结合伙伴进行平行筛选来推动生物学发展。同时，光刻方法能够实现每平方厘米合成超过50,000个寡核苷酸，这一进展彻底改变了整个基因组学领域。如果能获得价格合理、高密度的肽阵列，蛋白质组学领域也有望取得类似的发展。然而，肽阵列的发展落后于寡核苷酸阵列。本文综述了该领域的最新进展，重点介绍了能够实现超高密度肽阵列的方法。
