Brown Ninita H, Koreishi Anjum F, McCall Michelle, Izatt Joseph A, Rickman Catherine Bowes, Toth Cynthia A
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Graefes Arch Clin Exp Ophthalmol. 2009 Aug;247(8):1069-80. doi: 10.1007/s00417-009-1044-3. Epub 2009 Feb 19.
To compare spectral domain optical coherence tomography (SDOCT) cross-sectional images of human central retina obtained from donor eyes with and without age-related macular degeneration (AMD) to corresponding histopathology from light micrographs. To establish the utility of SDOCT for localizing pathology in the posterior eyecup, for identifying ocular disease in donor eyes, or for directing subsequent sectioning of retinal lesions for research.
Seven consecutive human donor eyes were selected based on age. The eyes, with the anterior segment removed, were imaged by SDOCT with a focusing aspheric lens. Four eyes were from donors with a clinical history of AMD, and three were from age-matched donors with no history of AMD. Histopathological correlation of morphological changes detected in three eyes by SDOCT was obtained for comparison to step serial-sectioned light microscopy images of the formalin-fixed, paraffin-embedded retina. A simplified imaging setup was tested on an enucleated porcine eye for comparison.
AMD pathology was detected and localized in four eyes by SDOCT. The SDOCT images correlated with the histopathology observed by light microscopy in each sectioned eye. Pathologies included a subfoveal neovascular lesion with subretinal fluid, peripapillary neovascularization, epiretinal membrane, foveal cyst, choroidal folds, and drusen. Similar imaging was possible with the simplified setup.
SDOCT imaging identified retinal disease of the posterior eyecup in human donor eyes. Pathology detected with SDOCT was verified by light microscopy in three eyes, supporting the utility of SDOCT as a screening tool for research.
比较从患有和未患有年龄相关性黄斑变性（AMD）的供体眼中获得的人中央视网膜的光谱域光学相干断层扫描（SDOCT）横截面图像与光学显微镜下相应的组织病理学图像。确立SDOCT在定位眼后杯病变、识别供体眼中的眼部疾病或指导后续视网膜病变切片用于研究方面的效用。
根据年龄连续选取7只人供体眼。去除前段后，用聚焦非球面透镜通过SDOCT对这些眼睛进行成像。4只眼来自有AMD临床病史的供体，3只眼来自年龄匹配且无AMD病史的供体。对SDOCT在3只眼中检测到的形态学变化进行组织病理学相关性分析，以与福尔马林固定、石蜡包埋视网膜的连续阶梯切片光学显微镜图像进行比较。在摘除的猪眼上测试了一种简化的成像装置以作比较。
通过SDOCT在4只眼中检测到并定位了AMD病变。SDOCT图像与每只切片眼的光学显微镜观察到的组织病理学结果相关。病变包括黄斑下新生血管病变伴视网膜下液、视乳头周围新生血管形成、视网膜前膜、黄斑囊肿、脉络膜皱褶和玻璃膜疣。使用简化装置也能进行类似成像。
SDOCT成像识别了人供体眼中眼后杯的视网膜疾病。在3只眼中通过光学显微镜证实了SDOCT检测到的病变，支持SDOCT作为一种研究筛查工具的效用。
